Description: CD59 Glycoprotein, Rat, mAb TH9
In rat, CD59 is expressed on vacular endothelium and circulating cells. In the central nervous system (CNS) of the rat, CD59 is expressed on the Schwann sheath of peripheral nerve fibres and on ependymal cells, but not on glial cells and neurons in the CNS. Rat astrocytes in vitro express CD59 on its surface.
CD59 may be involved in rheumatoid arthritis, motor nerve injury in the Guillain-Barré sydrome and in other diseases where defective inhibition of complement activation on self tissue is involved. Furthermore, CD59 may play an important part in abrogating the effects of complement attack in renal disease. Its presence and protective effect have already been demonstrated on human renal cells.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.|
|Application||F, FC, FS, W|
|Use||For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.|
|Immunogen||Partially purified (FPLC) rat CD59 prep (RIP)|
|Alias||Membrane attack complex inhibition factor, MACIF, MAC-inhibitory protein, Protectin, CD59|
|References||1. Hughes, T et al; Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement. Biochem J 1992, 284: 169
2. Hughes, T et al; Immunolocalization and characterization of the rat analogue of human CD59 in kidney and glomerular cells. Immunology. 1993, 80: 439
3. Rogers, C et al; Expression and function of membrane regulators of complement on rat astrocytes in culture. Immunology 1996, 88: 153
|Storage and stability||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Disease||Autoimmunity, Infectious diseases|