Technical datasheet
Description
Monoclonal antibody F1 binds both natural and recombinant mouse gamma Interferon. Its binding activity has been demonstrated in vitro and in vivo. F1 antibodies have been demonstrated to be able to inhibit inflammatory responses to bacterial lipopolysaccharides. These antibodies were furthermore shown to inhibit Shwartzman reactions and to protect NZB mice against spontaneous development of autoimmune disease. The neutralizing activity of the antibody has been demonstrated as being poor in anti-viral assays. If a neutralizing antibody is specifically desired, we recommend the use of another antibody available from Hycult Biotech, namely F3 (prod. code HM1005) which recognizes a different epitope on the murine gamma Interferon molecule and in contrast to F1, exhibits strong neutralizing activity. The antibody does not react with rat or human gamma interferon
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rat IgG2a
Application
The antibody can be used for the purification or immunochemical investigation of mouse gamma Interferon
and structural studies. Furthermore the antibody is useful for immunoprecipitation and as a tracer antibody
in immuno assay procedures.
Storage and stability
Lyophilized product should be stored at 4°C. Store stock solution in aliquots at -20°C. Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
References
1. Armstrong, JA et al; Semi-micro, dye-binding assay for rabbit interferon. J Appl Microbiol 1971,
21: 723
2. van Tiel, FH et al; Detection of Semliki Forest virus in cell culture by use of an enzyme immunoassay with peroxidase-labeled monoclonal antibodies specific for glycoproteins E1 and E2. J Clin Microbiol 1984, 20: 387
3. Dijkmans, R et al; Heterogeneity of Chinese hamster ovary cell-produced recombinant murine interferon-gamma. J Biol Chem 1987, 262: 2528
4. Heremans, H et al; Regulation by interferons of the local inflammatory response to bacterial lipopolysaccharide. J Immunol 1987, 138 : 4175
5. Billiau, A et al; anti-interferon-gamma antibody protects mice against the generalized Shwartzman reaction. Eur J Immunol 1987, 17 : 1851
6. Jacob, CO et al; in vivo treatment of (NZB X NZW) F1 lupus-like nephritis with monoclonal antibody to gamma interferon. J Exp Med 1987,
166: 798
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
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HM1002A
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IFN-gamma, Mouse, mAb F3
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HM1002B
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IFN-gamma, Mouse, mAb F3
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HM1003B
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IFN-gamma, Mouse, mAb F1
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HC1020A
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IFN-gamma, Mouse, Recombinant (CHO-Derived)
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HC1020B
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IFN-gamma, Mouse, Recombinant (CHO derived)
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