Name
IFN-gamma, Human, ELISA kit (medium & serum)
Catalog nr
HK309
(lot number and expiry date are indicated on the label)
Short description
Human interferons are grouped into three major classes: interferon alpha (IFN-alpha), interferon beta (IFN-beta) and interferon gamma (IFN-gamma). An extensive variety of biological effects have been observed for the interferon family including antiviral activity, antiproliferative activity, stimulation of cytotoxic activities in lymfocytes, natural killer cells and macrophages and modulation...
Size
2 x 96 det.
Application
Manual
Description
Human interferons are grouped into three major classes: interferon alpha (IFN-alpha), interferon beta (IFN-beta) and interferon gamma (IFN-gamma). An extensive variety of biological effects have been observed for the interferon family including antiviral activity, antiproliferative activity, stimulation of cytotoxic activities in lymfocytes, natural killer cells and macrophages and modulation of the major histocompatibility complex. IFN-gamma is produced by only two cell types: T-cells and larger granular lymphocytes. IFN-gamma probably exists in its native form as a dimer of the two active species of 20 and 25 kD, respectively.
Application
The Human IFN-gamma ELISA has been developed for the quantitative measurement of both natural and recombinant human IFN-gamma in serum, plasma, and cell culture medium. In serum or plasma samples, human IFN-gamma can be measured accurately if serum or plasma samples are diluted at least 10 times. Most reliable results are obtained if EDTA plasma is used.
Features
- Minimum concentration which can be measured is 20 pg/ml human IFN-gamma.
- Measurable concentration range of 20-5,000 pg/ml.
- Working volume of 100 µl/well.
Typical standard curve
Principle
- The human IFN-gamma ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
- The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay.
- Samples and standards are captured by a solid bound specific antibody.
- Biotinylated tracer antibody will bind to captured human IFN-gamma.
- Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
- Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
- The enzyme reaction is stopped by the addition of citric acid.
- The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human IFN-gamma standards (log).
- The human IFN-gamma concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least six months. After reconstitution the reagents are stable for 1 month if stored at 2-8°C.
References
- Weimer, R et al; HIV-induced IL-6/IL-10 dysregulation of CD4 cells is associated with defective B cell help and autoantibody formation against CD4 cells. Clin Exp Immunol 1998, 111: 20
- Micheletti, F et al; Supra-agonist peptides enhance the reactivation of memory CTL responses. J Immunol 2000: 165: 4264
- Audigé, A et al; HIV-1 does not provoke alteration of cytokine gene expression in lymphoid tissue after acute infection ex vivo. J Immunol. 2004, 172: 2687
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
- Weimer, R et al; HIV-induced IL-6/IL-10 dysregulation of CD4 cells is associated with defective B cell help and autoantibody formation against CD4 cells. Clin Exp Immunol 1998, 111: 20
- Micheletti, F et al; Supra-agonist peptides enhance the reactivation of memory CTL responses. J Immunol 2000: 165: 4264
- Audigé, A et al; HIV-1 does not provoke alteration of cytokine gene expression in lymphoid tissue after acute infection ex vivo. J Immunol. 2004, 172: 2687
MSDS
| Related products | Cat # | |
|---|---|---|
| TNF-alpha, Human, ELISA kit | HK307 | |
| IFN-gamma, Human, ELISA kit (serum) | HK030S | |