Technical datasheet
Description
This glycosylated interferon is constitutively produced by Chinese Hamster Ovary (CHO) cells. The CHO cells have been transformed by a plasmid containing mouse coding sequences for gamma interferon as well as for dihydrofolate reductase. Biochemical analysis of CHO-derived mouse gamma interferon and gamma interferon as produced by mouse splenocytes revealed no structural differences.
The major advantage of CHO-cell derived hi-pure recombinant mouse gamma interferon over natural mouse gamma interferon is the absence of contaminating lymphokines. This ensures that the effects observed in experimental results can be attributed exclusively to the hi-pure mouse gamma interferon used.
One unit of Hycult Biotech Mouse Gamma Interferon approximates the bioactivity contained in one unit of the mouse gamma interferon standard prepared by NIH (Gg02-901-533).
Formulation
Lyophilized product in PBS, 100 µg mouse albumin and 0.01% gelatin containing 1.0 x 106 Units. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).
Species
Mouse, CHO-derived
Use
For dilutions use protein stabilized phosphate buffered saline, pH 8. (Do not use fresh serum).
Storage and stability
Lyophilized product should be stored at 4°C. Store stock solution after reconstitution in aliquots at -20°C. Repeated freeze and thaw cycles and prolonged exposure to light will cause loss of activity. Under recommended storage conditions, product is stable for one year.
References
1. Dijkmans, R et al; Molecular cloning of murine interferon gamma (MuIFN-gamma) cDNA and its expression in heterologous mammalian cells. J Interferon Res 1985, 5: 511
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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