Technical datasheet
Description
This glycosylated interferon is constitutively produced by Chinese Hamster Ovary (CHO) cells transfected with the chromosomal gamma interferon gene of the rat. Purification is performed from serum-free culture medium by affinity chromatography using monoclonal antibodies to rat gamma interferon. This ultra pure product is intended for laboratory use in in vitro assay systems or in experimental animals. Biological activity has been demonstrated in both rat and murine systems.
The major advantage of CHO-cell derived recombinant rat gamma interferon over natural rat gamma interferon, is the absence of contaminating lymphokines. This ensures that the effects observed in experimental results can be attributed exclusively to the rat gamma interferon used.
One unit of Hycult Biotech Rat Gamma Interferon approximates the bioactivity contained in one unit of the rat gamma interferon standard prepared by the TNO laboratory.
Formulation
Lyophilized product in PBS and 0.01% gelatin containing 1.0 x 105 Units. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).
Species
Rat, CHO-derived
Use
For dilutions use protein stabilized phosphate buffered saline, pH 8. (Do not use fresh serum).
Storage and stability
Lyophilized product should be stored at 4°C. Store stock solution after reconstitution in aliquots at -20°C. Repeated freeze and thaw cycles and prolonged exposure to light will cause loss of activity. Under recommended storage conditions, product is stable for one year.
References
1. van der Meide, P et al; The purification and characterization of rat interferon by use of two monoclonal antibodies. J Gen Virol 1986, 67: 1059 2. Schellekens, H et al; Production and initial characterization of rat interferon. J Gen Virol 1980, 46: 243
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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