Technical datasheet
Description
The peptide Polymyxin B (PMB) is a cyclic amphipathic peptide antibiotic that binds to endotoxins (lipopolysaccharide, LPS). Binding of PMB to LPS results in neutralization of LPS of gram-negative bacteria. For the study of PMB-LPS interactions PMB conjugates proved to be superior to the application of anti-PMB antibodies. PMB conjugates are useful general probes to detect or measure lipid A and LPS of various species using simple methods. These conjugates are also useful for staining of bacteria. The conjugates obviate the need for many specific antisera. Furthermore PMB conjugates are useful tools in investigations of LPS binding protein (LBP) and bactericidal permeability increasing protein (BPI) interactions with LPS. In conclusion, PMB conjugates are useful probes for endotoxin research.
Formulation
0.2 ml biotinylated purified PMB in PBS, containing 0.1% BSA and 0.02% 2-chloroacetamide.
Application
Biotinylated PMB can be used in immuno assays.
Use
For immuno assays dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Storage and stability
Lyophilized product should be stored at 4°C. After reconstitution store stock solutions in aliquots at -20°C. Avoid repeated freezing and thawing. Under recommended storage conditions, product is stable for more than one year.
References
1. Appelmelk, B et al; Polymyxin B-horseradish peroxidase conjugates as tools in endotoxin research. Anal Biochem 1992, 207: 311
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
Appelmelk, B et al; Polymyxin B-horseradish peroxidase conjugates as tools in endotoxin research. Anal Biochem 1992, 207: 311