Technical datasheet
Description
The monoclonal antibody 18D11 recognizes human monocyte marker CD14, a 53 kDa glycoprotein. CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present at low density on a subpopulation granulocytes and activated or transformed B-cells.
The monoclonal antibody 18D11 reacts with both soluble and membrane CD14. The monoclonal antibody 18D11 is useful for inhibition of biological activity of sCD14, it inhibits LPS induced TNF production.
The monoclonal antibody is cross reactive with pig CD14. Together with a C5a neutralizing monoclonal antibody, 18D11 blocks sepsis in a pig model.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The monoclonal antibody 18D11 can be used for flow cytometry. Furthermore the monoclonal antibody 18D11 is useful for inhibition of biological activity of sCD14.
Use
For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of human sCD14 to be inactivated.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Tunheim, G et al; Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes. J Leukoc Biol 2005, 77: 303
- Wang, J et al; Peptidoglycan primes for LPS-induced release of proinflammatory cytokines in whole human blood. Shock 2001, 16: 178
- Wang, J et al; Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 2001 69: 2402
- Wang, J et al; Peptidoglycan and lipoteichoic acid from Staphylococcus aureus induce tumor necrosis factor alpha, interleukin 6 (IL-6), and IL-10 production in both T cells and monocytes in a human whole blood model. Infect Immun 2000 68: 3965
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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