Technical datasheet
Description
Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species.
Formulation
0.5 mg of 0.2 μm filtered antibody solution in PBS (exact concentration is indicated on the label), containing 0.02% sodium azide.
Species
Mouse IgG1
Application
The monoclonal antibody 3E7 can be used for blocking the complement-activating capacity of human MBL. The antibody 3E7 is useful for Western blotting and immuno assays. Furthermore the monoclonal antibody 3E7 is useful for immunohistology on frozen sections and flow cytometry. The antibody is not useful for staining of paraffin embedded sections.
Use
For Western blotting, flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity dilutions have to be made according to the amounts MBL to be inactivated.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Muto, S et al; Biological activities of human mannose-binding lectin bound to two different ligand sugar structures, Lewis A and Lewis B antigens and high-mannose type oligosaccharides. Biochimica et Biophysica Acta 2001, 1527: 39
- Matsushita, M et al; Human mannose-binding protein is identical to a component of Ra-reactive factor. Biochem Biophys Res Commun 1992, 183: 645
- Hisano, S et al; Mesangial IgA2 deposits and lectin pathway-mediated complement activation in IgA glomerulonephritis. Am J Kidney Dis 2001, 38: 1082
- Vries de, B et al; The mannose-binding lectin-pathway is involved in complement activation in the course of renal ischemia-reperfusion injury. Am J Pathol 2004, 165: 1677
- Nauta, A et al; Mannose-binding lectin engagement with late apoptotic and necrotic cells. Eur J Immunol 2003, 33 : 2853
- Nauta, A et al ; Opsonization with C1q and Mannose-binding lectin targets apoptotic cells to dendritic cells. J Immunol 2004, 173: 3044
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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