Technical datasheet
Description
Calprotectin, also known as MRP-8/MRP-14 or S100A8/A9 heterocomplex, is formed out of the calcium-binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9). The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is calcium-dependent. Calprotectin comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Furthermore, it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity. During intestinal wall inflammation, granulocytes transmigrate through the intestinal wall. Therefore calprotectin is also detectable in faeces. Several investigations report that faecal calprotectin is significantly increased in intestinal diseases such as inflammatory bowel disease (IBD), Crohn´s disease, ulcerative colitis and colon cancer. Normal human plasma contains a calprotectin concentration ranging from 500 to 3000 ng/ml.
Formulation
Lyophilized product in 74mM Tris-HCl and 104mM NaCl, pH7.4 containing 50 µg human Calprotectin. Reconstitute the vial by injection of 0.5 ml distilled or de-ionised water (Caution: vial is under vacuum).
Application
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F
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FC
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FS
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IA
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IF
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IP
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P
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W
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Yes
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●
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application notes
FS: Inhibition of bacterial growth – Test method is inhibition of S. Aureus
Storage and stability
Product should be stored at –70°C. Repeated freeze and thaw cycles will cause loss of activity. The exact expiry date is indicated on the label. Use recombinant Calprotectin protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.
References
- Hunter, M et al; High level expression and dimer characterization of the S100 EF-hand proteins, migration inhibitory factor-related proteins 8 and 14. J Biol Chem 1998, 273: 12427
- Yui, S et al; Implication of extracellular zinc exclusion by recombinant human calprotectin (MRP8 and MRP14) from target cells in its apoptosis-inducing activity. Mediators Inflamm 2002, 11: 165
- Corbin, B et al; Metal chelation and inhibition of bacterial growth in tissue abscesses. Science 2008, 319: 962
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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