Name
MPO, Mouse, ELISA kit
Catalog nr
HK210-01
(lot number and expiry date are indicated on the label)
Short description
Myeloperoxidase (MPO) is a glycoprotein with a alpha2beta2 heteromultimer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent o...
Size
1 x 96 det.
Application
Manual
Description
Myeloperoxidase (MPO) is a glycoprotein with a alpha2beta2 heteromultimer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. MPO is rapidly released by activated polymorphonuclear neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegeners disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention.
Cross Reactivity
Potential cross reacting proteins detected in the mouse MPO ELISA
|
Cross reactant |
Reactivity |
|
Rat MPO |
weak |
Application
The mouse MPO ELISA has been developed for the quantitative measurement of natural and recombinant mouse MPO in cell culture medium, plasma and tissue homogenates. In plasma samples MPO can be measured accurately if plasma samples are diluted at least 4 times. Most reliable results are obtained if Heparin plasma is used. Please be aware that mouse MPO is released from neutrophils into serum in the process of blood coagulation. This will lead to false positive and difficult to interpret results of serum samples. Therefore it is advised to use 'careful plasma'.
Features
- Minimum concentration which can be measured is 1.0 ng/ml mouse MPO.
- Measurable concentration range of 1.0-250 ng/ml.
- Working volume of 100 µl/well.
Typical standard curve
Principle
- The mouse MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
- The efficient format a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
- Samples and standards are captured by a solid bound specific antibody.
- Biotinylated tracer antibody will bind to captured mouse MPO.
- Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
- Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
- The enzyme reaction is stopped by the addition of citric acid.
- The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse MPO standards (log).
- The mouse MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least six months. After reconstitution the reagents are stable for 1 month if stored at 2-8°C. After reconstitution never store the standard below 0°C because even a single freeze and thaw cycle cause loss of MPO activity and give erroneous results.
Linearity
The linearity of the assay was determined by serially diluting a serum sample containing 49.62 ng/ml mouse MPO. The diluted samples were measured in the assay. The line obtained a slope of 1.03 and a correlation coefficient of 0.998. 
References
- Vries de, B et al; Exogenous alpha-1-acid glycoprotein protects against renal ischemia-reperfusion injury by inhibition of inflammation and apoptosis. Transplantation 2004, 78: 1116
- Xu, H et al; A coagulation factor Vll deficiency protects against acute inflammatory responses in mice. J Pathol 2006, 210: 488
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
- Vries de, B et al; Exogenous alpha-1-acid glycoprotein protects against renal ischemia-reperfusion injury by inhibition of inflammation and apoptosis. Transplantation 2004, 78: 1116
- Xu, H et al; A coagulation factor Vll deficiency protects against acute inflammatory responses in mice. J Pathol 2006, 210: 488
MSDS
| Related products | Cat # | |
|---|---|---|
| MPO, Human, ELISA kit | HK324-02 | |
| MPO, Rat, ELISA kit | HK105-02 | |