Technical datasheet
Description
The monoclonal antibody 266-6K1 recognizes human myeloperoxidase (MPO), an ~135 glycoprotein expressed in all cells of the myeloid linage. MPO functions as an α2β2 heteromultimer consisting of two heavy (α) and two light (β) chains of 55 and 15 kDa respectively. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils (PMNs). It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Upon activation of neutrophils, MPO can be rapidly released and as such useful in body fluids as marker for inflammatory status.
Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease, inflammatory bowel disease and multiple sclerosis. Autoimmune antibodies to MPO (so called ANCA) are involved in Wegener’s disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention.
Cross Reactivity
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Cross reactant
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Reactivity
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Rat
Mouse
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No
No
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Formulation
1 ml (100 µg/ml) 0.2 µm filtered FITC conjugated antibody solution in PBS, containing 0.02% sodium azide and 1% bovine serum albumin.
Species
Mouse IgG1
Application
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F
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FC
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FS
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IA
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IF
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IP
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P
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W1
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Yes
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●
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●
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No
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●
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N.D.
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●
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●
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●
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●
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application IA has been tested by Hycult Biotech.
Application notes
W: Samples were subjected to SDS-PAGE and transferred to PVDF membrane (Ref.1).
IA: The antibody can be used as coat and detector.
Use
For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Positive control
Neutrophils, HL-60 cells
Negative control
Erythrocytes
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. La Rocca, G et al; Oxidative stress induces myeloperoxidase expression in endocardial endothelial cells from patients with chronic heart failure. Basic Res Cardiol 2009, 104: 307
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
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HM2164BT
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MPO, Human, mAb 266-6K1, biotinylated
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HM2164
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MPO, Human, mAb 266-6K1
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HM2172
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Proteinase 3, Human, mAb PR3-G2
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HM2173
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Lactoferrin, Human, mAb 265-1K1
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HM2174
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Elastase, Human, mAb 265-3K1
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HM1051
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MPO, Mouse, mAb 8F4
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HM3030
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MPO, Rat, mAb 2D4
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HP9048
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MPO, Human, pAb
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References
1. La Rocca, G et al; Oxidative stress induces myeloperoxidase expression in endocardial endothelial cells from patients with chronic heart failure. Basic Res Cardiol 2009, 104: 307