Technical datasheet
Description
LPS binding protein (LBP) is an approximately 60 kDa acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on mononuclear phagocytes, leading to an 100-1,000-fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negative cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into lipoproteins which neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS or gram negative bacteria. The LBP-peptide, amino acids 86-99, binds lipid A and neutralizes LPS in vivo and in vitro.
Formulation
Lyophilized, purified LBP-peptide from human LBP protein in PBS. The exact peptide amount is indicated on the label. Reconstitute the vial by injection of sterile, endotoxin free phosphate buffered saline.
Application
LBP-peptide can be used in biological assays in vivo and in vitro.
Use
For neutralization of biological activity dilutions have to be made according to the amounts protein to be inactivated. Further dilutions should be made by using protein stabilized phosphate buffered saline, pH 7. (Never use fresh serum/plasma). Be aware: endotoxin will bind LBP-peptide.
Storage and stability
Lyophilized product should be stored at 4°C. The stock solution should be stored in aliquots at -70°C in polypropylene tubes. LBP-peptide stock solution should never be thawed more than once. Repeated freeze and thaw cycles will cause loss of LBP-peptide activity. Under recommended storage conditions, product is stable for more than one year.
References
- Bucki, R et al; Extracellular Gelsolin binds lipoteichoic acid and modulates cellular response to proinflammatory bacterial wall components. J of Immunol. 2008, 181: 4936
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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