Technical datasheet
Description
The monoclonal antibody 31 reacts specifically with secretory leukocyte proteinase inhibitor (SLPI; also known as antileukoprotease (ALP)). SLPI is a 11.7 kDa cationic inhibitor of neutrophil elastase and to a lesser extent of cathepsin G. It is locally produced by epithelial cells in the lung, skin and other organs and by Polymorphonuclear leukocytes (PMN) and (in mice) by macrophages.
In addition to its proteinase inhibitory properties that may serve to protect against proteolytic injury, it was recently shown that SLPI also displays several other functions such as antimicrobial and antiinflammatory activities. These appear to be independent of its ability to inhibit PMN serine proteinases. SLPI has also been demonstrated to display antibacterial and antifungal activity at concentrations in which SLPI is present in mucosal secretions including those of the lung. Another possible role for SLPI is inhibition of proteindisulphide isomerase that is considered essential for invasion of a cell by the Human Immunodeficiency Virus (HIV).
The reactivity of the antibody 31 with isolated domains of SLPI was evaluated using domains obtained by cleavage using partial acidic hydrolysis. Due to this fact the monoclonal antibody 31 recognizes other SLPI cleavage products.
Formulation
0.5 mg of 0.2 μm filtered antibody solution in PBS (exact concentration is indicated on the label), containing 0.02% sodium azide.
Species
Mouse IgG1
Application
The monoclonal antibody 31 can be used for immunohistology on frozen and paraffin sections. Furthermore the monoclonal antibody 31 is useful for Western blotting, immuno precipitation and immuno assays.
Use
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- De Water, R et al; Ultrastructural localization of bronchial antileukoprotease in central and peripheral human airways by a gold-labeling technique using monoclonal antibodies. Am Rev Resp Dis 1986, 133: 882
- Wingens, M et al; Induction of SLPI (ALP/HUSI-I) in epidermal keratinocytes. J Invest Dermatol 1998, 111: 996
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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