Name
Neutrophils, Mouse, mAb NIMP-R14
Catalog nr
HM1039
(lot number and expiry date are indicated on the label)
Short description
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although pr...
Size
100 µg
Application
F
,
FC
,
FS
,
IA
,
P
Technical datasheet
Description
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
Immunogen
Purified BALB/c mouse neutrophils
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Rat IgG2b
Application
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot Application FC has been tested by Hycult Biotech.
|
Application notes
IHC-F: Tissue was fixed with acetone (Ref 6)
IHC-P: Blocking with 20% normal rabbit serum (Ref 4)
FC: 5 x 105 cells were incubated with 10 µg/ml antibody (Ref 3)
FS: Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection (Ref 5)
Staining for Ly-6G/-6C of frozen section of mouse spleen
Use
For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Aliases
Lymphocyte antigen 6 complex locus protein G6c, myeloid differentiation antigen Gr1
Positive control
Mouse neutrophils
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Lopez, A et al; Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies, Brit J Haematology 1984, 57: 489
- McLaren, D et al; Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells. Parasite Immunol 1987, 9: 667
- Van Lent, P et al; Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice. J Leukoc Biol 1997, 61: 267
- Lubberts, E et al; Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction. J Immunol 1999, 163: 4546
- Tacchini-Cottier, F et al; An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major. J Immunol 2000, 165: 628
- Vries de, B et al; Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 2003, 170: 3883
- Nagendra, S et al; Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 2004, 58: 195
- Navarini, A et al; Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections. PNAS 2009, 106: 7107
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
| HM1039F | Neutrophils, Mouse, mAb NIMP-R14, FITC |
| HM1039BT | Neutrophils, Mouse, mAb NIMP-R14, biotinylated |
| HM1051 | MPO, Mouse, mAb 8F4 |
| HM1051BT | MPO, Mouse, mAb 8F4, biotinylated |
| HM1051F | MPO, Mouse, mAb 8F4, FITC |
References
- Lopez, A et al; Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies, Brit J Haematology 1984, 57: 489
- McLaren, D et al; Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells. Parasite Immunol 1987, 9: 667
- Van Lent, P et al; Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice. J Leukoc Biol 1997, 61: 267
- Lubberts, E et al; Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction. J Immunol 1999, 163: 4546
- Tacchini-Cottier, F et al; An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major. J Immunol 2000, 165: 628
- Vries de, B et al; Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 2003, 170: 3883
- Nagendra, S et al; Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 2004, 58: 195
- Navarini, A et al; Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections. PNAS 2009, 106: 7107
Protocols
Protocols
Protocols
Protocols
| Related products | Cat # | |
|---|---|---|
| MPO, Mouse, mAb 8F4, biotinylated | HM1051BT | |
| MPO, Mouse, mAb 8F4, FITC | HM1051F | |
| MPO, Mouse, mAb 8F4 | HM1051 | |
| Neutrophils, Mouse, mAb NIMP-R14, FITC | HM1039F | |
| Neutrophils, Mouse, mAb NIMP-R14, biotinylated | HM1039BT | |