Technical datasheet
Description
Cysteine-rich secretory protein 3 (CRISP-3; also known as SGP28) is a glycoprotein that belongs to the family of cysteine-rich secretory proteins (CRISPs) which was originally discovered in human neutrophilic granulocytes. CRISP-3 is also widely distibuted in exocrine glands (salivary glands, pancreas and prostate), eosinophilic granulocytes and to a lower level in epididymis, ovary, thymus and colon. The presence of CRISP-3 in neutrophils, eosinophils and in exocrine secretions indicates a role in innate host defense. The antibody has been raised against recombinant C-terminally truncated form of CRISP-3 and recognizes both the N-glycosylated and non-glycosylated form of the mature protein.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Rabbit IgG
Application
The antibody can be used for immuno assays, both as coating and detection, Western blotting and immunohistology on paraffin sections.
Use
For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Kjeldsen, L et al; SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with
similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein. FEBS Lett
1996, 380: 246
2. Udby, L et al; An ELISA for SGP28/CRISP-3, a cysteine-rich secretory protein in human neutrophils, plasma, and exocrine secretions. J Immunol Methods 2002, 263: 43
3. Udby, L et al; Identification of human cysteine-rich secretory protein 3 (CRISP-3) as a matrix protein in a subset of peroxidase-negative granules of neutrophils and in the granules of eosinophils. J Leukoc Biol 2002,
72: 462
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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References
1. Kjeldsen, L et al; SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with
similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein. FEBS Lett
1996, 380: 246
2. Udby, L et al; An ELISA for SGP28/CRISP-3, a cysteine-rich secretory protein in human neutrophils, plasma, and exocrine secretions. J Immunol Methods 2002, 263: 43
3. Udby, L et al; Identification of human cysteine-rich secretory protein 3 (CRISP-3) as a matrix protein in a subset of peroxidase-negative granules of neutrophils and in the granules of eosinophils. J Leukoc Biol 2002,
72: 462