Lactoferrin, Human, pAb - HP9034

Name

Lactoferrin, Human, pAb

Catalog nr

HP9034 (lot number and expiry date are indicated on the label)

Short description

Human lactoferrin (LF) is an 80 kDa glycoprotein which was first isolated from human milk. It plays an important part in the immune system and helps to fight infections. Lactoferrin promotes the health of the gastro-intestinal system by improving the intestinal microbial balance. In addition, LF can be found in epithelia and most body fluids and secretions. Lactoferrin is secreted in plas...

Size

100 µg

Application

IA , IP , W

Technical datasheet

HP9034.pdf

Description

Lactoferrin is secreted in plasma by neutrophils. Its plasma concentration also represents a positive relation to the total pool of neutrophils and the rate of neutrophil turnover. In inflammation lactoferrin is released from secondary granules of neutrophilic leukocytes into the extracellular medium. Therefore the extracellular lactoferrin concentration can be used as an index for neutrophil activation.

Lactoferrin strongly binds to iron and this iron binding property is considered to be an important antimicrobial. Human lactoferrin binds to bacterial products through its highly positively charged N-terminus, it kills various bacteria, most probably by inducing intracellular changes in these bacteria without affecting the membrane permeability. Cleavage by pepsin of lactoferrin leads to the release of lactoferricin H. This 47 amino acid peptide has more antimicrobial activity than its precursor and it can inhibit the classical but not the alternative complement pathway.

Lactoferrin also plays a role in signal transduction, immunomodulation and has antiadhesive, anticancer, antiviral activity.

Formulation

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

Species

Bovine IgG

Application

The antibody can be used for immuno assays, both as coating and detection, Western blotting and immuno precipitation.

Use

For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Storage and stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

References

1. Nuijens, J et al; Characterization of recombinant human lactoferrin secreted in milk of transgenic mice. J Biol Chem 1997, 272: 8802

2. Nibbering, P et al; Human lactoferrin and peptides derived from its N terminus are highly effective against infections with antibiotic-resistant bacteria. Infect Immun 2001, 69: 1469

3. Veen, van H et al; Analytical cation-exchange chromatography to assess the identity, purity, and N-terminal integrity of human lactoferrin. Anal Biochem 2002, 309: 60

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available

HM2173	Lactoferrin, Human, mAb 265-1K1
HM2037	SLPI, Human, mAb 31
HP9027	Elastase, Human, pAb
HP9033	CRISP-3, Human, pAb
HP9035	Lysozyme, Human, pAb

References

1. Nuijens, J et al; Characterization of recombinant human lactoferrin secreted in milk of transgenic mice. J Biol Chem 1997, 272: 8802

2. Nibbering, P et al; Human lactoferrin and peptides derived from its N terminus are highly effective against infections with antibiotic-resistant bacteria. Infect Immun 2001, 69: 1469

3. Veen, van H et al; Analytical cation-exchange chromatography to assess the identity, purity, and N-terminal integrity of human lactoferrin. Anal Biochem 2002, 309: 60

MSDS

MSDS purified antibodies with azide.pdf

Protocols

Protocol Immunoprecipitation.pdf

Protocols

Protocol Sandwich ELISA.pdf

Protocols

Protocol Western Blotting.pdf

Related products		Cat #
SLPI, Human, mAb 31		HM2037
Lactoferrin, Human, mAb 265-1K1		HM2173
Elastase, Human, pAb		HP9027
Lysozyme, Human, pAb		HP9035
CRISP-3, Human, pAb		HP9033