Human SAA ELISA
Wednesday - November 24, 2010
Human acute phase Serum Amyloid A protein, SAA, as inflammatory marker
Human SAA ELISA for detection in urine, plasma and serum
Human SAA and inflammation
§ Serum amyloid A (SAA) is predominantly produced by the liver
§ Extra-hepatic production by macrophages, endothelial cells, epithelial cells, artherosclerotic lesions, tumors and synovial tissue
§ Four different genes in human produce SAA. SAA-1 is the predominant form in plasma
§ SAA is a major acute phase protein
§ Belongs to family of pentraxins (i.e. PTX3, CRP)
§ SAA functions as chemo-attractant
Human SAA detection in urine
§ To exclude matrix influence, urine with low SAA (#15) and urine with higher SAA (#16) concentration were mixed in different ratios
§ The calculated concentration of human SAA was plotted versus the expected concentration of human SAA (Fig 1)
Evaluation of matrix-influence
§ To exclude matrix influence, several serum and urine samples were diluted and recovery was calculated (Fig 1)
§ Minimal dilutions of 5x and 50x are recommended for urine and serum, respectively
§ SAA is an early indicator for transplant rejection
§ Increase in human SAA within hours after bacterial as well as viral infection
§ predictor for coronary artery disease in women
§ SAA functions in various physiological and pathological processes
§ SAA is linked to inflammation, atherosclerosis, thrombosis, AA-amyloidosis, rheumatoid arthritis, and neoplasia.
§ sensitive marker of acute inflammatory state
§ Unique assay for measurement in urine
§ Human SAA concentration rises earlier and sharper than acute phase protein CRP
§ Human SAA is increased after bacterial as well as viral infection
§ Benchtop stability of standard > 95% (2 hrs RT; o/n 4 °C)
§ Working time of 2.5 hours
§ 100 µl sample/well
§ Detection limit 3.1 ng/ml
§ Useful for serum, plasma, fat-biopts and urine
§ Detection of natural SAA-1
§ Normal values in serum and plasma range from 1-5 µg/ml
§ Acute inflammation may results in 1000-fold increase in human SAA
Available related products
|
Cat. No |
Product |
|
Human LBP ELISA |
|
|
Human SLPI ELISA |
|
|
Human LL37 ELISA |
|
|
Human Pentraxin 3 ELISA |
More information on
Nate: Hycult Biotech's Mascot Read this article » Shock Conference, June 9th - 13th, Miami Beach, FL Read this article » New Antibody Sampler Packs US Read this article » New: Flow Cytometry Brochure! Read this article » NEW: Human Properdin ELISA Read this article » Low Endotoxin Antibodies for Functional Studies Read this article » BSA-free Antibodies for Immuno Assays Read this article » Now available: 1-plate assays! Read this article » Special Formulations and Service Read this article » 2012 US Hycult Biotech Catalog Read this article » Discounts for Publication References Read this article » Government Accounts Read this article » American Assoc. of Immunology Meeting Read this article » Human Properdin ELISA and mAbs Read this article » Immuno Assay mAbs Read this article » Single-Plate ELISAs Read this article » Functional Study, Low Endotoxin mAbs Read this article » PTX3, a biomarker for vascular inflammation Read this article » Upgrade I-FABP ELISA (Cat.#HK406) Read this article » Visit us at MEDICA Read this article » New R-PE monoclonals available Read this article » Hycult Biotech Inc Bronze Sponsor SLB Read this article » NEW product: Human CRP ELISA Read this article » Hycult Biotech Gold Sponsor EMCHD 2011 Read this article » Discount Human LBP ELISA and Human sCD14 ELISA Read this article » Variant-specific quantification of factor H Read this article » Increased Exposure to Bacterial Antigen RpL7/L12 Read this article » IgG glycan hydrolysis Read this article » Differential role of the lectin pathway Read this article » C1q deficiency linked to autoimmune disorders Read this article » New Complement Proteins available Read this article » Formation of Hycult Biotech Inc. Read this article » New Human Complement Factor D ELISA Read this article » Unique ELISA to elucidate the role of human MASP-3 Read this article » Mast cells, basophils and eosinophils Read this article » Unique antibodies for functional studies Read this article » C1q product release Read this article »