MGO-modified proteins, Human, mAb MGO-1 - HM5014

Name

MGO-modified proteins, Human, mAb MGO-1

Catalog nr

HM5014 (lot number and expiry date are indicated on the label)

Short description

The monoclonal antibody MGO-1recognizes human MGO modified proteins. Methylglyoxal (MGO) is an endogenous product of glucose metabolism. Increased production and accumulation of methylglyoxal ...

Size

100 µg

Application

IA , P , W

Technical datasheet

Description

The monoclonal antibody MGO-1recognizes human MGO modified proteins. Methylglyoxal (MGO) is an endogenous product of glucose metabolism. Increased production and accumulation of methylglyoxal (MGO), as well as increased modification of proteins by glycoxidation, are hallmarks of aging and diabetes. MGO was shown to modify proteins and to contribute to the accumulation of damaged proteins that can be toxic to cells. A number of studies have shown that MGO levels are significantly elevated in patients with Type 2 Diabetes and correlates well with fasting plasma glucose and hemoglobin A1c (HbA1c) levels. Moreover, increased formation of the MGO is implicated in renal dysfunction and is known to be involved in the development of DN (diabetic nephropathy).

Cross Reactivity

Cross reactant	Reactivity
Multispecies	Yes

Immunogen

MGO-modified KLH

Formulation

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide

Species

Mouse IgG₁

Application

	F	FC	FS	IA	IF	IP	P	W
Yes				●			●	●
No
N.D.	●	●	●		●	●

N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application P and W are based on personal communication.

Use

For immunohistochemistry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Storage and stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Also available

HM5013	CML, Human, mAb CML26
HM5001	Nitrotyrosine, mAb HM.11
HM5002	Nitrotyrosine, mAb HM.11, biotinylated
HP5002	Chlorotyrosine, pAb

MSDS

MSDS purified antibodies with azide.pdf

Protocols

Protocol Immunohistochemistry Paraffin.pdf

Protocols

Protocol SDS Page.pdf

Protocols

Protocol Western Blotting.pdf

Related products		Cat #
Nitrotyrosine, mAb HM.11		HM5001
Nitrotyrosine, mAb HM.11, biotinylated		HM5002
Chlorotyrosine, pAb		HP5002
CML, Human, mAb CML26		HM5013