Name
Macrophages F4/80, Mouse, mAb BM8, biotinylated
Catalog nr
HM1066BT
(lot number and expiry date are indicated on the label)
Short description
The monoclonal antibody BM8 recognises a 125 kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overal...
Size
50 µg
Application
F
,
FC
,
P
,
W
Technical datasheet
Description
The monoclonal antibody BM8 recognises a 125 kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny.
The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
The monoclonal antibody BM8 cross reacts with human heart macrophages.
Cross Reactivity
| Cross reactant | Reactivity (Yes/No) |
| Human heart macrophages | Yes |
| Mouse granulocytes | No |
| Mouse mast cells | No |
| Mouse platelets | No |
| Mouse lymphocytes | No |
| Mouse fibroblasts | No |
| Mouse endothelial cells | No |
Immunogen
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
Formulation
0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rat IgG2a
Application
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F 1,4 |
FC1,2 |
FS |
IA |
IF |
IP |
P3 |
W1 |
|
Yes |
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No |
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N.D. |
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
1-2 Applications are based on literature data and were not tested by Hycult Biotech.
Application notes
W: Mouse bone-marrow derived macrophages; non-reduced; ~125 kDa (Ref 1); reduction with 2-mercaptoethanol destroys BM8 antigen
F: tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control
P: fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control (Ref 3)
FC: fixed with 1 % paraformaldehyde (Ref 1)
F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8 (Cat. # HM1066).
F: tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control
P: fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control (Ref 3)
FC: fixed with 1 % paraformaldehyde (Ref 1)
F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8 (Cat. # HM1066).
Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections.
Positive control
Mouse macrophages
Negative control
Mouse fibroblasts or granulocytes
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Malorny, U et al; A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res 1986, 243: 421
2. Leenen, P et al; Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 1994, 174: 5
3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879
4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035
2. Leenen, P et al; Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 1994, 174: 5
3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879
4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
| HM1066 | Macrophages F4/80, Mouse, mAb BM8 |
| HM1066F | Macrophages F4/80, Mouse, mAb BM8, FITC |
| HM1061 | SR-A, Mouse, mAb 2F8 |
| HM1067 | Dectin-1, Mouse, mAb 2A11 |
| HM1068 | MARCO, Mouse, mAb ED31 |
References
1. Malorny, U et al; A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res 1986, 243: 421
2. Leenen, P et al; Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 1994, 174: 5
3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879
4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035
2. Leenen, P et al; Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 1994, 174: 5
3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879
4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035
Protocols
Protocols
Protocols
Protocols
| Related products | Cat # | |
|---|---|---|
| SR-A, Mouse, mAb 2F8 | HM1061 | |
| Macrophages F4/80, Mouse, mAb BM8 | HM1066 | |
| MARCO, Mouse, mAb ED31 | HM1068 | |
| Macrophages F4/80, Mouse, mAb BM8, FITC | HM1066F | |
| Dectin-1, Mouse, mAb 2A11 | HM1067 | |