Name
LBP, various species, ELISA kit
Catalog nr
HK503
(lot number and expiry date are indicated on the label)
Short description
Lipopolysacharide (LPS) Binding Protein (LBP) is a type 1 acute phase protein that is constitutively produced by the liver and rapidly upregulated during the acute phase response. LBP plays a central role in the response to LPS. The protein catalyses the monomerization of LPS and its transfer to (s)CD14 and to lipoproteins. In this way LBP has both a role in the activation pathway of LPS: act...
Size
1 x 96 det.
Application
Manual
Description
Lipopolysacharide (LPS) Binding Protein (LBP) is a type 1 acute phase protein that is constitutively produced by the liver and rapidly upregulated during the acute phase response. LBP plays a central role in the response to LPS. The protein catalyses the monomerization of LPS and its transfer to (s)CD14 and to lipoproteins. In this way LBP has both a role in the activation pathway of LPS: activation of monocytes by LPS leading to release of inflammatory mediators and in the neutralization of LPS i.e. the uptake of LPS by lipoprotein and subsequent clearing. The assay can be used to quantify functional LBP in tissue culture supernatants of cell cultures, plasma and serum samples of several species. In plasma of healthy individuals LBP is present at levels which increase during acute phase responses.
Application
The LBP (lipopolysaccharide binding protein) kit is intended for the relative quantitative measurement of natural and recombinant LBP of a wide variety of species in cell culture medium, plasma or serum. Measurable species are: bovine, canine, human, monkey, rabbit, rat, sheep and swine. For quantification human LBP is used as reference standard. In serum or plasma samples LBP can be measured accurately if serum or plasma samples are diluted at least 3 times; dilution depends on the species measured. Most reliable results are obtained if EDTA plasma is used.
Features
- Minimum concentration which can be measured is 1.6 ng/ml.
- Measurable concentration range of human LBP is 1.6 tot 100 ng/ml.
- Working volume of 100 µl/well.
Typical standard curve
Principle
- The LBP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
- The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
- Samples and standards are incubated in microtiter wells coated with antibodies recognizing LBP.
- Biotinylated LPS will bind to captured LBP.
- Streptavidin-peroxidase conjugate will bind to the biotinylated LPS.
- Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
- The enzyme reaction is stopped by the addition of citric acid.
- The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the LBP standards (log).
- The LBP concentration of samples, which are run concurrently with the human LBP standards, can be determined from the human LBP standard curve.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least six months. After reconstitution the reagents are stable for 1 month if stored at 2-8°C.
References
- Gucht, S et al; Porcine Reproductive and respiratory syndrome virus infection increases CD14 expression and Lipopolysaccharide-Binding Protein in the lungs of pigs. Viral Immunol 2005, 18: 116
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
- Gucht, S et al; Porcine Reproductive and respiratory syndrome virus infection increases CD14 expression and Lipopolysaccharide-Binding Protein in the lungs of pigs. Viral Immunol 2005, 18: 116
MSDS
| Related products | Cat # | |
|---|---|---|
| LBP, Human, ELISA kit | HK315-02 | |
| BPI, Human, ELISA kit | HK314 | |
| LBP, Mouse, ELISA kit | HK205 | |