Name
LBP, Mouse, ELISA kit
Catalog nr
HK205
(lot number and expiry date are indicated on the label)
Short description
Lipopolysaccharide (LPS) Binding Protein (LBP) is a type 1 acute phase protein that is constitutively produced by the liver and rapidly upregulated during the acute phase response. LBP plays a central role in the response to LPS. The protein catalyses the monomerization of LPS and its transfer to (s)CD14 and to lipoproteins. In this way LBP has both a role in the activation pathway of LPS: ac...
Size
2 x 96 det.
Application
Manual
Description
Lipopolysaccharide (LPS) Binding Protein (LBP) is a type 1 acute phase protein that is constitutively produced by the liver and rapidly upregulated during the acute phase response. LBP plays a central role in the response to LPS. The protein catalyses the monomerization of LPS and its transfer to (s)CD14 and to lipoproteins. In this way LBP has both a role in the activation pathway of LPS: activation of monocytes by LPS leading to release of inflammatory mediators and in the neutralization of LPS i.e. the uptake of LPS by lipoprotein and subsequent clearing. The assay can be used to quantify LBP in tissue culture supernatants of mouse cell cultures and in plasma and serum samples. In plasma of normal mice LBP is present at levels of approx. 6 μg/ml, which increase approx. 10-fold during acute phase responses.
Application
The mouse LBP ELISA has been developed for the quantitative measurement of natural mouse LBP in cell culture medium, plasma or serum. In serum or plasma samples, mouse LBP can be measured accurately if serum or plasma samples are diluted at least 500 times. Most reliable results are obtained when EDTA plasma is used.
Features
- Minimum concentration which can be measured is 0.4 ng/ml mouse LBP.
- Measurable concentration range of 0.4 -100 ng/ml.
Typical standard curve
Principle
- The mouse LBP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4½ hours.
- The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay.
- Samples and standards are captured by a solid bound specific antibody.
- Biotinylated tracer antibody will bind to captured LBP.
- Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
- Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
- The enzyme reaction is stopped by the addition of citric acid.
- The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse LBP standards (log).
- The mouse LBP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least six months. After reconstitution the reagents are stable for 1 month if stored at 2-8°C, except for the standard. After reconstitution, the standard is stable for 2-3 days. For longer stability, we recommend to store aliquots at -20 °C.
References
- Branger, J et al; LPS-binding protein-deficient mice have an impaired defense against Gram-negative but not Gram-positive pneumonia. Int Immunol 2004, 16: 1605.
- Tiirola, T et al; Effect of acute Chlamydia pneumoniae infection on lipoprotein metabolism in NIH/S mice. Scand J Clin Lab Invest 2002, 62: 477.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
- Branger, J et al; LPS-binding protein-deficient mice have an impaired defense against Gram-negative but not Gram-positive pneumonia. Int Immunol 2004, 16: 1605.
- Tiirola, T et al; Effect of acute Chlamydia pneumoniae infection on lipoprotein metabolism in NIH/S mice. Scand J Clin Lab Invest 2002, 62: 477.
MSDS
| Related products | Cat # | |
|---|---|---|
| ENDOBLOCK LBP ELISA kit | HIT301 | |
| MBL-A, Mouse, ELISA kit | HK208 | |
| MBL-C, Mouse, ELISA kit | HK209 | |