Technical datasheet
Description
The monoclonal antibody EfB1-3 recognizes the mouse ephrin receptors EphB1, EphB2 and EphB3, 118 kDa, 110 kDa and 110 kDa, respectively.
Erythropoietin-producing human hepatocellular carcinoma (Eph) receptors and ephrins are membrane proteins. They are classified into two subclasses, namely A and B, according to structural characteristics and binding specificities. Eph receptors are tyrosine kinases, which autophosphorylate upon binding to their cognate ephrin ligands. Eph receptors and ephrins are frequently expressed in reciprocal patterns that correlate with cellular boundaries during embryonic development.
A unique feature of Eph-ephrin interactions is that they can facilitate bi-directional communication between cells. Eph receptors and ephrin ligands are involved in various animal development processes, and implicated in some cancers. Eph-ephrin interactions mediate a variety of contact-dependent phenomena in both the developing and mature central nervous system. They are involved in neural crest cell migration, hindbrain segmentation, morphogenetic moments during gastrulation, brain guidance in the inner ear, retinal ganglion cell axon guidance and axon bundling in the hippocampus.
The monoclonal antibody EfB1-3 can also be used to stain cultured spinal cord explants.
Cross Reactivity
| Cross reactant |
Reactivity |
| Rat |
Yes |
| Chicken |
Yes |
| Zebra fish |
Yes |
Immunogen
Rat EphB1-Fc
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
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F
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FC
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FS
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IA
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IF
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IP
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P
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W
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|
Yes
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Use
For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Tyrosine-protein kinase receptor EPH-2, HEK6, ELK
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Jevince, A et al; Distribution of EphB receptors and ephrin-B1 in the developing vertebrate spinal cord. J Comp Neurol 2006, 497: 734
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
References
1. Jevince, A et al; Distribution of EphB receptors and ephrin-B1 in the developing vertebrate spinal cord. J Comp Neurol 2006, 497: 734