Technical datasheet
Description
The FITC conjugated monoclonal antibody C6G recognizes both smoothelin-A and smoothelin-B. Its epitope is located within a stretch of 30 amino acids in exon 11 (a.a. 56-86 of smoothelin-A, 512-542 of smoothelin-B). Smoothelin is one of the smooth muscle cell (SMC) specific proteins such as calponin, alpha smooth muscle cell actin, SM22alpha and smooth muscle myosin heavy chain isoforms. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. It colocalizes with actin stress fibers, this distribution indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Two major isoforms of smoothelin have been reported, a 59-kDa smoothelin-A in visceral smooth muscle cells and a 110-kDa smoothelin-B in vascular smooth muscle cells. Both gene products are encoded by a single copy gene which is located on chromosome 22 and regulated by a dual promoter system in a tissue specific manner. The variation in the smoothelin proteins is achieved by alternative splicing in the calponin homology domain. Antibodies to smoothelin are powerful tools in evaluation of smooth muscle cell differentiation as occurs in atherosclerotic lesions.
Cross Reactivity
| Cross reactant |
Reactivity |
| Goat |
Yes |
| Rabbit |
Yes |
| Dog |
No |
| Pig |
No |
| Mouse |
No |
Immunogen
Recombinant human smoothelin-A
Formulation
1 ml (100 µg/ml) 0.2 µm filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide
Species
Mouse IgG1
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | ● | ● | | | ● | | | ● | | No | | | | | | | | | | N.D. | | | ● | ● | | ● | ● | | |
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot |
Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Smoothelin-A, short smoothelin, smoothelin-B
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Rensen, S et al; Expression of the smoothelin gene is mediated by alternative promoters. Cardiovasc Res 2002, 55: 850
- Saleem, M et al; The molecular and functional phenotype of glomerular podocytes reveals key features of contractile smooth muscle cells. Am J Physiol Renal Physiol 2008, 295: F959
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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