CD200R3, Mouse, mAb Ba91, FITC - HM1104F

Name

CD200R3, Mouse, mAb Ba91, FITC

Catalog nr

HM1104F (lot number and expiry date are indicated on the label)

Short description

The monoclonal antibody Ba91 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38 kDa or ~74 kDa (reduced or non-reduced condition, respectively). CD200R3 presumably exists as a disulfide-linked dimer on the cell surface. The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R contains two Ig-like extracellular domains and mediates inhibitory s...

Size

100 µg

Application

Technical datasheet

HM1104F.pdf

Description

The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R contains two Ig-like extracellular domains and mediates inhibitory signals in myeloid cells. Mice deficient for the ligand CD200 develop enhanced experimental allergic encephalomyelitis and collagen-induced arthritis. Unlike CD200R, the CD200R-like receptors functions as activating receptors. They contain short cytoplasmic tails and a lysine residue in the transmembrane region and are likely to signal via adaptor proteins such as DAP12, DAP10, FcRg, or CD3z.

CD200R3 is expressed preferentially on cells of the myeloid lineage, including mast cells and basophils. Both cell types are involved in the host defense system against pathogens and in the development of allergic disorders. They function as independent essential initiators of allergic reactions. Basophils are the least common leukocytes in the peripheral blood accounting approximately 0.5 % of all leukocytes. In vivo depletion of basophils by using monoclonal antibody proved the essential role of basophils in the development and maintenance of IgE-mediated chronic allergic inflammation.

The activating CD200 receptors, like CD200R3, play an important role in IgE-independent mast cell and basophil activation. Monoclonal antibody Ba91 activates basophils ex vivo and elicits systemic anaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibody Ba91 induces degranulation. Monoclonal antibody Ba91 is also useful for depletion of basophils.

Immunogen

Mouse primary basophils

Formulation

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Species

Rat IgG2a

Application

The FITC conjugated monoclonal antibody Ba91 can be used for flow cytometry.

Use

For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Aliases

CD200R3, CD200RLb, OX-2 Receptor 3

Storage and stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

References

Kojima, T et al; Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner. J Immunol 2007, 179: 7093

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.

Also available

HM1066	Macrophages F4/80, Mouse, mAb BM8
HM1071	Dendritic cells, Mouse, mAb MIDC-8
HM1082	Ly-6C, Mouse, mAb ER-MP20
HM1086	Fibroblasts, Mouse, mAb ER-TR7
HM1103	CD200R3, Mouse, mAb Ba103
HM1104	CD200R3, Mouse, mAb Ba91

References

Kojima, T et al; Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner. J Immunol 2007, 179: 7093

MSDS

MSDS FITC antibodies with azide.pdf

Protocols

Protocol Flow cytometry.pdf

Related products		Cat #
Fibroblasts, Mouse, mAb ER-TR7		HM1086
Ly-6C, Mouse, mAb ER-MP20		HM1082
Dendritic cells, Mouse, mAb MIDC-8		HM1071
Macrophages F4/80, Mouse, mAb BM8		HM1066
CD200R3, Mouse, mAb Ba91		HM1104
CD200R3, Mouse, mAb Ba103		HM1103