Technical datasheet
Description
The monoclonal antibody ER-MP21 recognizes CD71, the transferrin receptor 1. CD71 is a 200 kDa glycoprotein composed of two identical, disulfide-linked chains. Each chain is capable of binding transferrin, an iron-transport molecule. Binding of transferrin to its receptor followed by endocytosis of the receptor-ligand complex is a major cellular iron-uptake mechanism. Iron uptake by the proliferating cell is essential for the iron-containing enzyme ribonucleotide reductase involved with DNA synthesis. CD71 is not only expressed by cycling cells, but also by cells that require iron for other iron-dependent proteins, such as the enzyme peroxidase in monocytes. Rapidly proliferating cells, as in malignancy, generally express CD71 abundantly. Furthermore, CD71 has been described as a marker of immature, proliferating T cells. The monoclonal antibody ER-MP21 inhibits proliferation and differentiation during early T cell development. ER-MP21 recognizes murine CD71, but does not compete with transferrin binding.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 1% bovine serum
Species
Rat IgG2a
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | ● | ● | ●a | | | | | | | No | | | | | | | ● | | | N.D. | | | | ● | ● | ● | | ● | |
a = Inhibition of biological activity N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot |
Use
For immunohistology, and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Aliases
TFRC, TFR1, T9, p90
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year
References
- Leenen, P et al; Differential inhibition of macrophage proliferation by anti-transferrin receptor antibody ER-MP21: correlation to macrophage differentiation stage. Exp Cell Res 1990, 189: 55
- Brekelmans, P et al; Transferrin receptor expression as a marker of immature cycling thymocytes in the mouse. Cell Immunol 1994, 159: 331
- Brekelmans, P et al; Inhibition of proliferation and differentiation during early T cell development by anti-transferrin receptor antibody. Eur J Immunol 1994, 24: 2896
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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