Technical datasheet
Description
The bacterial pathogen Staphylococcus aureus is insensitive to antimicrobial host defense peptides such as defensins, protegrins, platelet microbicidal proteins and bacteriocins. Staphylococci have developed various resistance mechanisms including those specific for bacteriocins and several host defense peptides. A protein belonging to the resistance mechanism of Staphylococcus aureus is known as CHIPS (Chemotaxis Inhibiting Protein of Staphylococcus aureus). CHIPS is a protein produced by Staphylococcus aureus that inhibits chemotaxis of neutrophils by blocking the Formyl Peptide Receptor (FPR) and C5a Receptor on neutrophils. CHIPS and antibodies against CHIPS can be useful for various experimental infection models of Staphylococcus aureus. Furthermore these reagents can be of help in studies on the role of FPR and C5a in inflammatory processes. Monoclonal antibody JNC1 reacts with the N-terminus of CHIPS.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.01% sodium azide and 0.1% BSA.
Species
Mouse IgG1
Application
The antibody can be used for immuno assays, flow cytometry, Western blot and immunohistology on frozen and paraffin sections. Furthermore the antibody can be used for inhibition of biological activity.
Use
For immunohistology, Western blot and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10 for flow cytometry and 1:100 for Western blot and immunohistology. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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