Technical datasheet
Description
Carcinoma associated antigen MAM-6 also known as a milkfat globule membrane antigen is a mucus glycoprotein of >400 kD that is located mainly in the glycocalyx of most glandular epithelial cells. It is also located in the cytoplasm and on the membrane of most carcinoma cells and some other tumor cells. Antibody 115D8 recognizes epitope "a" of MAM-6 and reacts very strongly with a majority of carcinomas (99.2%) and adenomas (61.4%). It occasionally shows a very weak cross reactivity with sarcomas and leukemias. It does not react with normal intestine or colorectal adenomas, but reacts with colorectal carcinomas.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG2b
Application
The antibody can be used for immunoprecipitation, Western blotting and immunohistology on frozen and paraffin sections.
Use
For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Tsubura, A et al; Expression of MAM-3 and MAM-6 antigens in endometrial and endocervical adenocarcinomas. Virchows Arch A Pathol Anat Histopathol 1985, 407: 59
- Zotter, S et al; Immunohistochemical localization of the epithelial marker MAM-6 in invasive malignancies and highly dysplastic adenomas of the large intestine. Lab Invest 1987, 57: 193
- Yamada, D et al; Immunohistochemical expression of MAM-3 and MAM-6 antigens in salivary gland tumours. Virchows Arch A Pathol Anat Histopathol 1989, 415: 509
- Shibuya, C et al; Immunohistochemical study of a monoclonal antibody 115D8 against human milk-fat globule membrane (MAM-6) in some histological types of breast cancer. Nippon Geka Hokan 1990, 59: 295
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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