Technical datasheet
Description
The monoclonal antibody G-81 recognizes a fragment of human dermcidin (DCD). The antibiotic peptide dermcidin (110 amino acids) is specifically and constitutively expressed in the sweat glands. DCD is localized to the dark mucous cells of normal eccrine sweat glands. DCD is normally expressed in eccrine sweat glands and is expressed in cutaneous mixed tumours. DCD-immunopositive cells are expressed in the inner cells of the tubular and ductal structures.
The monoclonal antibody G-81 was produced against a sweat-specific antigen. It reacts to sweat but not to other body fluids (e.g. serum, saliva, semen, milk, urine and tears) in ELISA. The N-terminal amino acid sequence (18 amino acids residues) is identical to a part of human cachexia-associated protein and of a survival-promoting peptide for neuronal cells. Besides this, the sequence is identical to the C-terminal peptide (amino acids 62-79) of DCD. The monoclonal antibody G81 can be used as a specific marker for eccrine glands in the human skin.
The monoclonal antibody G-81 is useful as a immunohistochemical tool to find the tumour cells that differentiate towards eccrine secretory cells in cutaneous mixed tumours. Furthermore, the antibody can be used for the detection of biochemical and histochemical changes of the sweat gland in the healing process of wound.
Immunogen
Fractioned sweat peptides in complete Freund's adjuvant
Formulation
1 ml (> 200 µg/ml) culture medium with a low endotoxin level containing 0.02% sodium azide.
Species
Mouse IgM
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | | | | ● | | | ● | ●a | | No | | | | | | | | | | N.D. | ● | ● | ● | | ● | ● | | | |
a = monoclonal antibody reacts strongly to a 7 kDa band and faintly to 20, 27 and 33 kDa bands of sweat protein N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot |
Use
For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
AIDD, PIF, DSEP, HCAP, DCD-1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Sagawa, K et al; Production and characterization of a monoclonal antibody for sweat-specific protein and its application for sweat identification. Int J Legal Med 2003, 117: 90
- Minami, Y et al; Immunohistochemical staining of cutaneous tumours with G-81, a monoclonal antibody to dermcidin. Br J Dermatol 2004, 151: 165
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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