Technical datasheet
Description
Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Keratin 7 (K7) is expressed in a wide range of epithelial structures in humans. K7 occurs in the columnar and glandular epithelium of the lung, cervix, breast, in bile ducts, collecting ducts in the kidney and in mesothelium, but is absent in gastrointestinal epithelium, hepatocytes, proximal and distal tubules of the kidney and myoepithelium. The antibody OVTL 12/30 is an excellent tool in the differential diagnosis of malignant cells and to determine primary tumor localization. It stains a 54 kD protein band of cytoskeletal proteins from cell lines in one and two dimensional immunoblots.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The antibody can be used for immunohistology on paraffin embedded tissue and Western blotting.
Use
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- van Niekerk, C et al; Marker profile of mesothelial cells versus ovarian carcinoma cells. Int J Cancer 1989, 43:1065
- Ramaekers, F et al; Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 1990, 136:641
- van Niekerk, C et al; Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues. J Pathol 1991, 165:145
- van Niekerk, C et al; Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas. Am J Pathol 1991, 138:455
- van de Molengraft, F et al; OV-TL 12/30 (keratin 7 antibody) is a marker of glandular differentiation in lung cancer. Histopathology 1993, 22:35
- Smedts, F et al; Detection of keratin subtypes in routinely processed cervical tissue: implications for tumour classification and the study of cervix cancer aetiology. Virchows Arch 1994, 425:145
- Baars, J et al; The applicability of a keratin 7 monoclonal antibody in routinely Papanicolaou-stained cytologic specimens for the differential diagnosis of carcinomas. Am J Clin Pathol 1994, 101:257
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available