Technical datasheet
Description
Melanoma associated antigen (MAA) is dispersed in the cytoplasma of melanoma cells, and is more concentrated inside vacuoles and sometimes on the melanosomes. Occasionally the antigen is seen on the cell surface. The antigen is actively shed from living cells. Although the antigen is associated with melanomas, it is not codistributed with the tyrosinase activity associated with melagonesis. The antigen shows codistribution with cathepsin D, which is a marker for lysosomal functions. The antibody NKI/beteb recognizes a (pre)melanosomal 100 kD and 7 kD antigen (glycoprotein). The antibody reacts with melanomas, clear cells sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. Except for one case of non-Hodgkin's lymphoma in which macrophages were positive, no reactions with other tumors or tissues have been observed.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG2b
Application
The antibody can be used for immunofluorescence and immunohistology on frozen and paraffin sections. Formalin fixation and embedding in paraffin may affect the reactivity of the antibody.
Use
For immunofluorescence and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Vennegoor, C et al; A monoclonal antibody specific for cells of the melanocyte lineage. Am J Pathol 1988, 130: 179
- Le Poole, I et al; Presence or absence of melanocytes in vitiligo lesions: an immunohistochemical investigation. J Invest Dermatol 1993, 100: 816
- Adema, G et al; Melanocyte lineage-specific antigens recognized by monoclonal antibodies NKI-beteb, HMB-50, and HMB-45 are encoded by a single cDNA. Am J Pathol 1993, 143: 1579
- Adema, G et al; Molecular characterization of the melanocyte lineage-specific antigen gp100. J Biol Chem 1994, 269: 20126
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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