Technical datasheet
Description
Melanoma associated antigen (MAA) is dispersed in the cytoplasma of melanoma cells, and is more concentrated inside vacuoles and sometimes on the melanosomes. Occasionally the antigen is seen on the cell surface. The antigen is actively shed from living cells. Although the antigen is associated with melanomas, it is not codistributed with the tyrosinase activity associated with melagonesis. The antigen shows codistribution with cathepsin D, which is a marker for lysosomal functions. This antibody NKI/M6 recognizes a high molecular weight proteoglycan with a molecular weight of >450 kD (chondroitin sulfate) and 250 kD (core protein). The antibody NKI/M6 reacts with melanoma cells derived from cell lines and short term cultures and reacts preferentially with melanoma cells in frozen sections. NKI/M6 can also be used to detect melanoma lesions in vivo. NKI/M6 shows cross-reactivity with most naevi and perineurium, and shows weak reactivity with hair follicles.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The antibody can be used for immunohistology on frozen sections.
Use
For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- de Vries, J et al; Characterization of melanoma-associated surface antigens involved in the adhesion and motility of human melanoma cells. Int J Cancer 1986, 38:465
- Natali, P et al; Structural properties and tissue distribution of the antigen recognized by the monoclonal antibody 653.40S to human melanoma cells. J Natl Cancer Inst 1981, 67:591
- Buraggi, G et al ; Imaging with 131I-labeled monoclonal antibodies to a high-molecular-weight melanoma-associated antigen in patients with melanoma: efficacy of whole immunoglobulin and its F(ab')2 fragments. Cancer Res 1985, 45:3378
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available