Technical datasheet
Description
The monoclonal antibody 3F1 recognizes mouse poliovirus receptor, also named CD155. CD155 is expressed on cells of hematopoietic origin and by endothelial vessels inside secondary lymphoid organs including HEV. Upon activation, dendritic cells, T and B cells upregulate the surface level of CD155. CD155 is an ~ 45 kDa member of a subfamily of immunoglobulin-like adhesion receptors. Nectin 1-4 are other members of this subfamily, functioning in cell-cell contact by forming heterophilic and hemophilic trans-dimers. In contrast to nectins, CD155 lacks self adhesion potential but can interact with other nectins. This way, CD155 contributes in the formation of adherens junctions between endothelial and epithelial cells. Due to this adhesive capacity, CD155 influences processes such as cell motility, migration, growth, and formation of adherens junctions. Furthermore, CD155 is involved in immunological processes such as CD8 T cell-mediated tumor rejection, and NK cell-mediated killing. Many tumor types display an aberrant up-regulation of CD155 making them vulnerable to destruction by NK or cytotoxic T cells.
In human, poliovirus, vitronectin and several other cellular receptors were identified as ligands that bind to CD155. CD96 (TACTILE) and CD226 (DNAM-1), both members of the Ig-superfamily, are other ligands of human CD155. Murine CD155 does not serve as a functional poliovirus receptor, however, murine CD155 recognizes the same panel of ligands like nectin-3, CD96, CD226 and vitronectin. CD155-deficient mice develop normally, however, a reduced humoral immune response to antigens entering the organism via the gastrointestinal tract is shown.
Cross Reactivity
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Cross reactivity
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Cross reactant
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Reactivity
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Human
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No
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Immunogen
Ectodomain of recombinant His-tagged CD155
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Rat IgG2a
Application
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F
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FC1
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FS2
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IA
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IF
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IP
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P
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W
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Yes
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application notes
FC: Antibody clone 3F1 stains the extracellular domain of mouse CD155. The thymocytes were blocked with 5% mouse serum before incubation with clone 3F1 for 1 hour at 4°C As positive control CD4+CD8+ thymocytes was used and as negative control CD155-/- derived cells (Ref.1).
FS: Antibody clone 3F1 functions as antagonist. The antibody was functionally tested by blocking adhesion of Sf9 cells expressing mCD155 to RMA, CD4+ and CD8+ cells. Furthermore, antibody clone 3F1 prevenst formation of mCD155/ligand complexes (Ref.2).
Use
For immuno assays, immuno precipitation, immunohistology, flow cytometry and functional studies dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Poliovirus receptor, Pvr, nectin-like protein 5, Tage4
Positive control
CD4+CD8+ mouse thymocytes
Negative control
CD155-/- cells
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Maier, M. et al; The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens. Eur J Immunol 2007, 37: 2214
2. Seth, S et al; The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochem Biophys Res Commun. 2007, 364: 959
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
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HM1105
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CD96, Mouse, mAb 6A6
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HM1050
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JAM-A, Mouse, mAb BV12
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HM1052
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Nectin-2, Mouse, mAb 502-57
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HM1053
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Nectin-3, Mouse, mAb 103-A1
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HM1056
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JAM-C, Mouse, mAb 19H36
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References
1. Maier, M. et al; The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens. Eur J Immunol 2007, 37: 2214
2. Seth, S et al; The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochem Biophys Res Commun. 2007, 364: 959