Name
CD200R3, Mouse, mAb Ba91
Catalog nr
HM1104-FS
(lot number and expiry date are indicated on the label)
Short description
The monoclonal antibody Ba91 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38 kDa or ~74 kDa (reduced or non-reduced condition, respectively). CD200R3 presumably exists as a disulfide-linked dimer on the cell surface. The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R contains two Ig-like extracellular domains and mediates inhibitory s...
Size
0.5 mg
Application
FC
,
FS
,
IP
Technical datasheet
Description
The monoclonal antibody Ba91 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38 kDa or ~74 kDa (reduced or non-reduced condition, respectively). CD200R3 presumably exists as a disulfide-linked dimer on the cell surface.
The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R contains two Ig-like extracellular domains and mediates inhibitory signals in myeloid cells. Mice deficient for the ligand CD200 develop enhanced experimental allergic encephalomyelitis and collagen-induced arthritis. Unlike CD200R, the CD200R-like receptors functions as activating receptors. They contain short cytoplasmic tails and a lysine residue in the transmembrane region and are likely to signal via adaptor proteins such as DAP12, DAP10, FcRg, or CD3z.
CD200R3 is expressed preferentially on cells of the myeloid lineage, including mast cells and basophils. Both cell types are involved in the host defense system against pathogens and in the development of allergic disorders. They function as independent essential initiators of allergic reactions. Basophils are the least common leukocytes in the peripheral blood accounting approximately 0.5 % of all leukocytes. In vivo depletion of basophils by using monoclonal antibody proved the essential role of basophils in the development and maintenance of IgE-mediated chronic allergic inflammation.
The activating CD200 receptors, like CD200R3, play an important role in IgE-independent mast cell and basophil activation. Monoclonal antibody Ba91 activates basophils ex vivo and elicits systemic anaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibody Ba91 induces degranulation. Monoclonal antibody Ba91 is also useful for depletion of basophils.
Immunogen
Mouse primary basophils
Formulation
0.5 mg (100 µg/ml) 0.2 µm filtered antibody solution in PBS (exact concentration is indicated on the label)
Species
Rat IgG2a
Application
The monoclonal antibody Ba91 can be used for flow cytometry, and immuno precipitation. Furthermore, the monoclonal antibody Ba91 is useful for functional studies.
Use
For flow cytometry, and immuno precipitation, ilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Aliases
CD200R3, CD200RLb, OX-2 Receptor 3
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Kojima, T et al; Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner. J Immunol 2007, 179: 7093
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Also available
| HM1066 | Macrophages F4/80, Mouse, mAb BM8 |
| HM1071 | Dendritic cells, Mouse, mAb MIDC-8 |
| HM1082 | Ly-6C, Mouse, mAb ER-MP20 |
| HM1086 | Fibroblasts, Mouse, mAb ER-TR7 |
| HM1103 | CD200R3, Mouse, mAb Ba103 |
| HM1103F | CD200R3, Mouse, mAb Ba103, FITC |
| HM1104F | CD200R3, Mouse, mAb Ba91, FITC |
References
- Kojima, T et al; Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner. J Immunol 2007, 179: 7093
Scientific info
Mast cells, basophils and eosinophils in innate immunity
Basophils, mast cells and eosinophils are white blood cells involved in the innate immune response especially associated with allergy and asthma. These cells are characterized by the presence of lobulated nuclei and secretory granules in the cytoplasm. Upon stimulation these granules release their contents (degranulation), predominantly mediators and cytokines. They are key effector cells in IgE-associated immune response and participate in the innate immune responses to many pathogens, including bacteria and viruses.
Basophils
Although basophils constitute less than 1 % of circulating blood leukocytes, they gain more recognition as key players in the immune system. Basophils are often recruited to the site of allergic inflammation. Furthermore, basophils are candidate effector cells for the immune response against parasites and are a major source of the typical Th2 cytokine IL-4 during parasite infections.
The unavailability of mouse basophil-specific antibodies has long hampered the progress in clarifying the critical roles of basophils. Monoclonal antibodies Ba91 and Ba103 recognize the CD200 receptor type 3 (CD200R3). CD200R3 functions as an activating receptor on mast cells and basophils to regulate IgE-independent immune responses in cooperation with an inhibitory receptor CD200R. mAbs Ba91 and Ba103 selectively stain basophils from the peripheral blood, spleen, and bone marrow and mast cells form the peritoneal cavity and skin, but not from the intestine. In vitro incubation of Ba91 or Ba103 together with basophils or mast cells results in degranulation of the cells. Interestingly, intravenous injection of Ba103 into mice results in depletion of basophils but not mast cells.
In conclusion, mAbs Ba91 and Ba103 are usefool tools to study basophil function in a variety of mice species.
Mast cells
Like basophils and eosinophils, mast cells originate from hematopoietic stem cells in the bone marrow. However, they complete their differentiation in the peripheral tissues. Mast cells have been implicated in rheumatoid arthritis, multiple sclerosis, atherosclerosis, inflammatory bowel disease and angiogenesis.
Eosinophils
Eosinophils are granulocytes that develop during hematopoiesis in the bone marrow before migrating into blood. They constitute ~1-3 % of the total white blood cells and are characterized by specific cytoplasmic granules that contain a series of cationic toxins able to kill many targets, including helminths, protozoa, bacteria and other cells. In bronchial asthma, predominantly major basic protein (MBP) is released resulting in tissue abnormalities. The number of these cells (eosinophil count) increases in certain illnesses, including allergies, asthma, Addison's disease, sarcoidosis, parasite infections, drug reactions, and connective tissue diseases (such as rheumatoid arthritis and scleroderma).
Basophils, mast cells and eosinophils are white blood cells involved in the innate immune response especially associated with allergy and asthma. These cells are characterized by the presence of lobulated nuclei and secretory granules in the cytoplasm. Upon stimulation these granules release their contents (degranulation), predominantly mediators and cytokines. They are key effector cells in IgE-associated immune response and participate in the innate immune responses to many pathogens, including bacteria and viruses.
Basophils
Although basophils constitute less than 1 % of circulating blood leukocytes, they gain more recognition as key players in the immune system. Basophils are often recruited to the site of allergic inflammation. Furthermore, basophils are candidate effector cells for the immune response against parasites and are a major source of the typical Th2 cytokine IL-4 during parasite infections.
The unavailability of mouse basophil-specific antibodies has long hampered the progress in clarifying the critical roles of basophils. Monoclonal antibodies Ba91 and Ba103 recognize the CD200 receptor type 3 (CD200R3). CD200R3 functions as an activating receptor on mast cells and basophils to regulate IgE-independent immune responses in cooperation with an inhibitory receptor CD200R. mAbs Ba91 and Ba103 selectively stain basophils from the peripheral blood, spleen, and bone marrow and mast cells form the peritoneal cavity and skin, but not from the intestine. In vitro incubation of Ba91 or Ba103 together with basophils or mast cells results in degranulation of the cells. Interestingly, intravenous injection of Ba103 into mice results in depletion of basophils but not mast cells.
In conclusion, mAbs Ba91 and Ba103 are usefool tools to study basophil function in a variety of mice species.
Mast cells
Like basophils and eosinophils, mast cells originate from hematopoietic stem cells in the bone marrow. However, they complete their differentiation in the peripheral tissues. Mast cells have been implicated in rheumatoid arthritis, multiple sclerosis, atherosclerosis, inflammatory bowel disease and angiogenesis.
Eosinophils
Eosinophils are granulocytes that develop during hematopoiesis in the bone marrow before migrating into blood. They constitute ~1-3 % of the total white blood cells and are characterized by specific cytoplasmic granules that contain a series of cationic toxins able to kill many targets, including helminths, protozoa, bacteria and other cells. In bronchial asthma, predominantly major basic protein (MBP) is released resulting in tissue abnormalities. The number of these cells (eosinophil count) increases in certain illnesses, including allergies, asthma, Addison's disease, sarcoidosis, parasite infections, drug reactions, and connective tissue diseases (such as rheumatoid arthritis and scleroderma).
| Related products | Cat # | |
|---|---|---|
| Fibroblasts, Mouse, mAb ER-TR7 | HM1086 | |
| Ly-6C, Mouse, mAb ER-MP20 | HM1082 | |
| Dendritic cells, Mouse, mAb MIDC-8 | HM1071 | |
| Macrophages F4/80, Mouse, mAb BM8 | HM1066 | |
| CD200R3, Mouse, mAb Ba103 | HM1103 | |
| CD200R3, Mouse, mAb Ba103, FITC | HM1103F | |
| CD200R3, Mouse, mAb Ba91, FITC | HM1104F | |