Technical datasheet
Description
The monoclonal antibody 6A6 recognizes mouse CD96. CD96 or T cell-Activated Increased Late Expression (TACTILE) is an ~67 kD immunoglobulin-like protein expressed on natural killer (NK) and T cells. CD96 belongs to the Ig-super family, consisting of three Ig-like domains and an extended serine/threonine/praline-rich stalk in the ectodomain. CD96 is upregulated upon T cell activation and is involved in engagement of diseased cells and cell movement towards areas of inflammation. Mouse CD96 binds to CD155 and nectin-1. CD96/CD155-interaction is preserved between man and mouse although both receptors are only moderately conserved in amino acid sequence. The monoclonal antibody 6A6 completely inhibits binding of CD96 to both recombinant mouse CD155 and mouse nectin-1. Also, T cell adhesion to insect cells expressing mCD155 is blocked by this monoclonal antibody, however, depending on the T cell subtype. The monoclonal antibody 6A6 was raised after immunization of rats with purified recombinant mCD96-hIgG1.
Cross Reactivity
| Cross reactant |
Reactivity (Yes/No) |
| Mouse |
Yes |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Species
Rat IgG2a
Application
The monoclonal antibody 6A6 can be used for immuno assay and Western blot. Furthermore, the monoclonal antibody 6A6 is useful for flow cytometry and functional studies.
Use
For Western blotting and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of CD96 to be inactivated.
Aliases
TACTILE
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Seth, S et al; The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochem Biophys Res Commun 2007, 364: 959
- Maier, M et al; The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens. Eur J Immunol 2007, 37: 2214
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
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HM1052
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Nectin-3, Mouse, mAb 103-A1
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HM1056
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JAM-C, Mouse, mAb 19H36
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HM1106
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CD155, Mouse, mAb 3F1
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HM2210
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CD96, Human, mAb NK92.39
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