MHC class II, Mouse, mAb ER-TR3 - HM1087

Innate Immunity

Inflammation

Cell- and tissue damage

Name

MHC class II, Mouse, mAb ER-TR3

Catalog nr

HM1087 (lot number and expiry date are indicated on the label)

Short description

Size

100 µg

Application

F , FC

Technical datasheet

HM1087.pdf

Description

The monoclonal antibody ER-TR3 reacts with mouse major histocompatibitity complex class II (MHC class II), also known as I-A or I-E. MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen-presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they acquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC Class II.

The monoclonal antibody ER-TR3 is a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells and macrophages) and for studying the development of T helper cells since they stain stromal cells in thymus. The level of antigen detected by ER-TR3 differs from strain to strain. The monoclonal antibody ER-TR3 cross reacts with human MHC class II on tonsil.

Distribution of ER-TR3 among mouse strains with independent and recombinant haplotypes.

Strain	Haplotype							Clone
	B	A	B	J	E	C	D	ER-TR3*
C3H/HeJ	k	k	k	k	k	k	k	46
AKR	k	k	k	k	k	k	k	54
B10.BR	k	k	k	k	k	k	k	62
B10.ScSn	b	b	b	b	b	b	b	50
Balb/b	b	b	b	b	b	b	b	39
B10.D2/n	d	d	d	d	d	d	d	54
Balb/C	d	d	d	d	d	d	d	44
DBA/2	d	d	d	d	d	d	d	47
B10.G	q	q	q	q	q	q	q	46
DBA/1	q	q	q	q	q	q	q	54
SWR/J	q	q	q	q	q	q	q	49
A.SW	s	s	s	s	s	s	s	6
B10.M	f	f	f	f	f	f	f	3
B10.RIII	r	r	r	r	r	r	r	40
B10.AQR	q	k	k	k	k	d	d	51
B10.T(6R)	q	q	q	q	q	q	d	52
A.TL	s	k	k	k	k	k	d	51
A.TH	s	s	s	s	s	s	d	7

* Percentage of labeled cells, determined by FACS analysis of spleen cell suspension

Cross Reactivity

Cross reactant	Reactivity (Yes/No)
Human	Yes

Formulation

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Species

Rat IgG2b

Application

The monoclonal antibody ER-TR3 can be used for immunohistology on frozen sections. Furthermore the monoclonal antibody ER-TR3 is useful for flow cytometry.

Use

For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Storage and stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

References

Van Vliet, E et al; Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol 1984, 14: 524
Van Vliet, E et al; Stromal cell types in the developing thymus of the normal and nude mouse embryo. Eur J Immunol 1985, 15: 675

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available

HM1071	Dendritic cells, Mouse, mAb MIDC-8
HM1066	Macrophages F4/80, Mouse, mAb BM8
HM1081	MGL, Mouse, mAb ER-MP23
HM1080	SIGN-R1, Mouse, mAb ER-TR9

References

Van Vliet, E et al; Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol 1984, 14: 524
Van Vliet, E et al; Stromal cell types in the developing thymus of the normal and nude mouse embryo. Eur J Immunol 1985, 15: 675

MSDS

MSDS purified antibodies with azide.pdf

Protocols

Protocol Immunohistochemistry Frozen.pdf

Protocols

Protocol Flow cytometry.pdf

Related products		Cat #
MGL, Mouse, mAb ER-MP23		HM1081
SIGN-R1, Mouse, mAb ER-TR9		HM1080
Dendritic cells, Mouse, mAb MIDC-8		HM1071
Macrophages F4/80, Mouse, mAb BM8		HM1066