Technical datasheet
Description
The monoclonal antibody ER-MP58 recognizes an antigen present on all mouse macrophage colony-stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells ut to the monocytic level, but also on grnaulocytes. ERxpression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commenly used myeloid markers as Mac-1, F4/80, and Gr-1. The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hemopoietic islands in various organs, and for embryonal studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting using monoclonal antibody ER-MP58 in combination with monoclonal antibody ER-MP12 (HM1084) and ER-MP-20 (HM1082).
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rat IgG2a
Application
The monoclonal antibody ER-MP58 can be used for immunohistology on frozen sections. Furthermore, the monoclonal antibody ER-MP58 is useful for flow cytometry.
Use
For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Cook, A et al; The effect of tissue type-plasminogen activator deletion and associated fibrin(ogen) deposition on macrophage localization in peritoneal inflammation. Thromb Haemost 2006, 95: 659
- Sunderkötter, C et al; Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response. J Immunol 2004, 172: 4410
- Nikolic, T et al; Developmental stages of myeloid dendritic cells in mouse bone marrow. Int Immunol 2004, 15: 515
- Chan, J et al; Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression. Blood 1998, 92: 1423
- De Bruijn, M et al; High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage. Eur J Immunol 1996, 26: 2850
- Leenen, P et al; Murine macrophage precursor characterization II. Monoclonal antibodies against macrophage precursor antigens. Eur J Immunol 1990, 20: 27
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
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