Technical datasheet
Description
The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.
Cross Reactivity
| Cross reactant |
Reactivity |
| Human |
Yes |
Immunogen
Crude rat liver membrane extracts
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
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F1-3,5
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FC4
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FS
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IA3,5
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IF,7
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IP
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P
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W2,4, 6
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Yes
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application notes
W: A non-reduced sample treatment and SDS-Page was used. The band size is 42 kDa (ref.4)
F: aceton fixed tissue section were incubated Hybridoma culture supernatant
W: 10% SDS-PAGE followed by blotting on nitrocellulose or PDFM, block with 10% non-fat dry milk
IA: microtiter plates were coated with 100µl 20ug/ml for ELISA
Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Aliases
ASGPR 1; ASGP-R 1; Hepatic lectin 1; MHL-1
Positive control
Rat hepatocytes
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Mizuno, M et al: Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Japan 1986, 21: 238
2. Mizuno, M et al; Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver 1987, 7: 251
3. Hyodo, I et al; Distribution of asioalogycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80
4. Shimada, M et al; A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res 2003, 26: 55
5. Hirai, M et al. Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody. Acta med okayama 2002 56: 135
6. Keyel, P et al. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol boil Cell 2006 17:4300
7. Zhou, X et al. Effects of hepatitis B virus S protein on human sperm function. Hum Reprod 2009 1: 1
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
1. Mizuno, M et al: Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Japan 1986, 21: 238
2. Mizuno, M et al; Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver 1987, 7: 251
3. Hyodo, I et al; Distribution of asioalogycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80
4. Shimada, M et al; A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res 2003, 26: 55
5. Hirai, M et al. Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody. Acta med okayama 2002 56: 135
6. Keyel, P et al. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol boil Cell 2006 17:4300
7. Zhou, X et al. Effects of hepatitis B virus S protein on human sperm function. Hum Reprod 2009 1: 1