Technical datasheet
Description
Dipeptidyl peptidase IV (DPP IV) is widely distributed in a number of mammalian tissues and is suggested to play an important role in various kinds of biological processes. DPP IV (CD26) is a serine-type protease that removes the amino-terminal dipeptide from peptide substrate provided that the penultimate amino acid residue is proline or alanine. DPP IV plays an important role in the reclamation of peptide nitrogen from larger peptides. The monoclonal antibody 5E8 reacts with DPP IV present on the apical surface of epithelial cells in the pancreas, small intestine, colon, and bile duct. Furthermore antibody 5E8 reacts with DPP IV on the laminar portions of the proximal renal tubule cells, and, weakly, on the glomeruli.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The monoclonal antibody 5E8 can be used for Western blotting, as detection in immuno assay and immuno precipitation. Furthermore the antibody 5E8 is very useful for immunohistology on frozen sections.
Use
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Mizuno, M et al; Monoclonal antibodies indentifying antigens on distinct domains of rat hepatocytes. Liver 1987, 7: 251
- Hyodo, I et al; Distribution of asialoglycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80
- Ariyoshi, M et al; Identification of a target antigen recognized by mouse monoclonal antibody to the bile canalicular surface of rat hepatocytes with a random phage display library. Acta Med Okayama 2002, 56: 187
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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