Name
Peripheral myelin protein 22, Human, mAb CF1
Catalog nr
HM2219
(lot number and expiry date are indicated on the label)
Short description
The monoclonal antibody CF1 recognizes human peripheral myelin protein 22 (PMP22). PMP22 is a 160 amino acid glycoprotein and contains four hydrophobic domains, presumably transmembrane regions. It is the second most abundant protein in the mammalian nervous system, but is, at least in rodents, also found in other tissues, including the liver and gut. Myelin protein zero (P0) is the most abunda...
Size
100 µg
Application
IA
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P
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W
Technical datasheet
Description
The monoclonal antibody CF1 recognizes human peripheral myelin protein 22 (PMP22). PMP22 is a 160 amino acid glycoprotein and contains four hydrophobic domains, presumably transmembrane regions. It is the second most abundant protein in the mammalian nervous system, but is, at least in rodents, also found in other tissues, including the liver and gut. Myelin protein zero (P0) is the most abundant protein in mammalian peripheral nerve myelin. An interaction between P0 and PMP22 has been detected in compact myelin. Defects in PMP22 have been related to Charcot-Marie-Tooth disease (CMT) and inflammatory demyelinating neuropathy (IDP). Immunization with PMP22 induces experimental auto-immune neuritis without central nervous inflammation. Therefore, PMP22 is considered as candidate auto-antigen in inflammatory diseases of the peripheral nervous system, including both acute and chronic inflammatory demyelinating polyradiculoneuropathy. Monoclonal antibody CF1 is crossreactive with Rhesus monkey PMP22.
Cross Reactivity
| Cross reactant | Reactivity |
| Human | Yes |
| Rhesus monkey | Yes |
Immunogen
Peptide corresponding to amino acids 121-133 in the second extracellular domain of human PMP2 with an extra N-terminal cysteine residue (CRHPEWHLNSDYSYG)
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG1
Application
The monoclonal antibody CF1 can be used for immuno assays and Western blotting. Furthermore, the monoclonal antibody CF1 is useful for immunohistology on paraffin embedded sections..
Use
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Growth arrest-specific protein 3, Gas-3
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Gregson, N et al; Characterization of a monoclonal antibody specific for human peripheral myelin protein 22 and its use in immunohistochemical studies of the fetal and adult nervous system. J Peripher Nerv Syst 2007, 12: 2
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Also available
References
- Gregson, N et al; Characterization of a monoclonal antibody specific for human peripheral myelin protein 22 and its use in immunohistochemical studies of the fetal and adult nervous system. J Peripher Nerv Syst 2007, 12: 2
Scientific info
PMP22 as marker for intact peripheral myelin sheats
Peripheral myelin protein 22 (PMP22) is the second most abundant protein in the mammalian nervous system, but is, at least in rodents, also found in other tissues, including the liver and gut. PMP22 is a 160 amino acid glycoprotein and contains four hydrophobic domains, presumably transmembrane regions. In compact myelin, PMP22 interacts with Protein 0 (P0, the most abundant myelin protein).
Defects in PMP22 have been related to Charcot-Marie-Tooth disease (CMT) and inflammatory demyelinating neuropathy (IDP). Immunization with PMP22 induces experimental auto-immune neuritis without central nervous inflammation.
PMP22 is considered as candidate auto-antigen in inflammatory diseases of the peripheral nervous system, including both acute and chronic inflammatory demyelinating polyradiculoneuropathy.
Figure: PMP22 staining in human paraffin embedded formalin fixed nerve section. Peripheral nerve myelin sheats are stained with monoclonal antibody CF1
Monoclonal antibody CF1 recognizes both human and rhesus monkey peripheral myelin protein 22, but is not useful for studying mouse PMP22. It can be used for immuno assays, Western blotting, and immunohistology on paraffin embedded sections.
Peripheral myelin protein 22 (PMP22) is the second most abundant protein in the mammalian nervous system, but is, at least in rodents, also found in other tissues, including the liver and gut. PMP22 is a 160 amino acid glycoprotein and contains four hydrophobic domains, presumably transmembrane regions. In compact myelin, PMP22 interacts with Protein 0 (P0, the most abundant myelin protein).
Defects in PMP22 have been related to Charcot-Marie-Tooth disease (CMT) and inflammatory demyelinating neuropathy (IDP). Immunization with PMP22 induces experimental auto-immune neuritis without central nervous inflammation.
PMP22 is considered as candidate auto-antigen in inflammatory diseases of the peripheral nervous system, including both acute and chronic inflammatory demyelinating polyradiculoneuropathy.
Figure: PMP22 staining in human paraffin embedded formalin fixed nerve section. Peripheral nerve myelin sheats are stained with monoclonal antibody CF1
Monoclonal antibody CF1 recognizes both human and rhesus monkey peripheral myelin protein 22, but is not useful for studying mouse PMP22. It can be used for immuno assays, Western blotting, and immunohistology on paraffin embedded sections.
Protocols
Protocols
Protocols