Technical datasheet
Description
The polyclonal antibody reacts with human Myeloperoxidase (MPO), a glycoprotein with an alpha2beta2 heteromultimer expressed in all cells of the myeloid lineage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. MPO is rapidly released by activated polymorphonuclear neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegeners disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention. In experimental studies antibodies to MPO can be used for various purposes ranging from flow cytometric analysis to detection of polymorphonuclear neutrophils in tissue sections. The polyclonal antibody is cross-reactive with mouse and rat MPO.
Cross Reactivity
| Cross reactant |
Reactivity |
| Rat |
Yes |
| Mouse |
Yes |
Formulation
1 ml 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Rabbit IgG
Application
The polyclonal antibody can be used for immuno assays, immunohistology on frozen and paraffin sections.
Use
For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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