Technical datasheet
Description
The monoclonal antibody 1.5D7 recognizes the disease associated isoform of the prion protein termed PrPSC. Prion diseases, also known as spongiform encephalopathies, are a group of neurodegenerative diseases that include BSE (bovine spongiform encephalopathy) in cattle, scrapie in sheep and CJD (Creutzfeldt-Jakob disease) in humans. The normal cellular form of the prion protein is denoted as PrPSC and is a constitutively expressed glycosylphosphatidylinositol anchored protein that has been shown to play a role in myelin formation. PrPSC has an unstructured N-terminal part and a C-terminal part consisting of three α -helices and two short β strands. Refolding of the normal prion protein results in PrPSc, which has a tightly packed C-terminal part enriched in beta sheets which is insoluble and resistant to digestion by proteases. Prion diseases are characterized by the deposition of highly structured aggregates of PrPSC, astrocytosis, neuronal cell death and spongiform structures in the brain. These diseases can be sporadically (unknown cause), be inherited due to polymorphisms or mutations in the prion protein gene or be transmitted by an infectious particle which is believed to consist of PrPSC only. In order to study prion diseases the detection of PrPSC and the ability to discriminate between the normal and disease associated PrP is of pivotal importance. The monoclonal antibody 1.5D7 can be used for the specific identification and characterization of PrPSC in tissue sections by immunohistochemistry and PET-blot.
Cross Reactivity
| Cross reactant |
Reactivity |
| Human |
Yes |
| Mouse |
Yes |
| Bovine |
Yes |
| Hamster |
Yes |
| Ovine |
Yes |
Immunogen
BoPrP153-165 (GSDYEDRYYRENM)
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG2b
Application
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F1
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FC2
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FS
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IA
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IF
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IP
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P
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W
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Yes
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application IA has been tested by Hycult Biotech.
Application notes
W: A reduced sample treatment and SDS-Page was used. The band size) is 30 kDa (Ref.1).
IHC-P: Paraffin sections were deparaffinized, rehydrated and endogenous peroxidase was quenched using 0.3% H2O2 in methanol for 20 min. As positive control BSE infected brain tissue was used and as negative control non-diseased brain tissue (Ref.1).
Use
For immunohistochemistry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Spongiform encephalopathies
Positive control
Prion diseased Brain tissue
Negative control
Non-diseased Brain tissue
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label
References
1. Cordes, H et al. Characterisation of new monoclonal antibodies reacting with prions from both human and animal brain tissues. J Immunol Method 2008, 337: 106.
2. Bergström, A et al; Short-term study of the uptake of PrPSc by the Peyer’s patches in hamsters after oral exposure to scrapie. J Comp Pathol 2006, 134: 126
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
References
1. Cordes, H et al. Characterisation of new monoclonal antibodies reacting with prions from both human and animal brain tissues. J Immunol Method 2008, 337: 106.
2. Bergström, A et al; Short-term study of the uptake of PrPSc by the Peyer’s patches in hamsters after oral exposure to scrapie. J Comp Pathol 2006, 134: 126