Description: Mannose receptor, Mouse, mAb MR5D3
MR’s are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IL-4. Labelling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. Detection of the MR with anti-MR monoclonal antibody MR5D3 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin|
|Application||F, FC, FS, IA, IF, IP, W|
|Application Notes||FC: 2% paraformaldehyde fixed cells were labeled with 10 µg/ml of mAb.
IHC-F:Sections fixed in ethanol or 2% paraformaldehyde were permeabilized with PBS/0.1% triton X100. After quenching and blocking mAb was added (10 µg/ml) and incubated for 1h
IA: mAb was used as detector at 10 µg/ml for 2h.
IP:MR was immunoprecipiated using antibody MR5D3 (10 µg/ml) and GammaBind Plus sepharose (Ref. 2)
W: Cleared lysates were run on 6% SDS-PAGE under nonreducing conditions. After transfer blot was stained with mAb ( 2 µm/ml).
FS: Antibody MR5D3 was used as a MR ligand to target MR+ dendtritic cells resulting in anti-rat IgG production (Ref. 6).
|Use||For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting.|
|Immunogen||Chimaeric CRD4-7-Fc protein|
|Alias||CD206, MMR, cLEC13D, Macrophage mannose receptor 1|
|References||1. Zamze, S et al; Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor. J Biol Chem 2002, 277: 41613
2. Martinez-Pomares, L et al. Analysis of mannose receptor regulation by IL4, IL10 an proteolytic processing using novel monoclonal antibodies. J. of Leuk Biol 2003, 73:604
3. Hassan, M et al. The schistosoa mansoni hepatic egg granuloma provides a favourable microenvironment for sustained growth of leishmania donovani. Am J Path 2006, 169:943
4. Duspasquir, M et al. The dermal microenvironment induces the expression of the alternative activation marker CD301/mGL in mononuclear phagocytes, independent of IL4/IL13 signalling. J Leuk Biol 2006, 80:838
5. Boskovic, J et al. Structural model for the mannose receptor family uncovered by electron microscopy of endo 180 and the mannose receptor. J Biol Chem 2006, 281:8780
6. McKenzie, E et al. Mannose receptor expression and function define a new population of murine dendritic cells. J immunol 2007, 178:4975
7. Miller, J et al. The mannose receptor mediates dengue virus infection of microphages. Plos pathogens 2008, 4:0001
8. Segura, E et al. Different cross-presentation pathways in steady-state and inflammatory dendritic cells. PNAS 2009 Dec 1;106:20377
9.-Hardison, S et al. Interleukin-17 is not required for classical macrophage activation in a pulmonary mouse model of Cryptococcus neoformans infection. Infect&immune 2010, 78:5341
10.-Geier, H et al. Phagocytic receptors dictate phagosomal escape and intracellular proliferation of francisella tularensis. Infect Immun 2011, march 21
|Storage and stability||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Disease||Cardiology and metabolism, Infectious diseases|