||1 x 96 det.
||78.1 to 5000 pg/ml
||RNase7, Ribonuclease 7, Skin-derived antimicrobial protein 2
||The human RNase 7 ELISA kit is to be used for the in vitro quantitative determination of human RNase 7 in serum, plasma, bronchoalveolar lavage fluid, urine and cell culture supernatant samples.
||Infectious diseases, Nephrology
||The human RNase 7 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human RNase 7. Biotinylated tracer antibody will bind to the captured human RNase 7. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human RNase 7 standards (log). The human RNase 7 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
|Storage and stability
||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
||1. Köten B, Simanski M, Gläser R, Podschun R, Schröder J-M, et al. (2009) RNase 7 Contributes to the Cutaneous Defense against Enterococcus faecium. PLoS ONE 4(7): e6424
2. Schwaderer A, Wang H, Bartz J, Kline J, Eichler T, DeSouza K, Sims-Lucas S, Baker P, D (2013) Ribonuclease 7, an antimicrobial peptide up-regulated during infection, contributes to microbial defense of the human urinary tract. Kidney Int. 83(4): 615–625.
3. Becknell B, Eichler T, Beceiro S, Li B, Easterling R, Carpenter A, James C, McHugh K, Hains D, Partida-Sanchez S, Spencer J. (2014) Ribonucleases 6 and 7 have antimicrobial function in the human and murine urinary tract. Kidney Int. 2014 Jul 30