0 items | $0.00

SPLUNC1, Mouse, pAb

Catalog #: HP8043

Quantity: 100 µg

Availability: In stock

Description: SPLUNC1, Mouse, pAb

The polyclonal antibody recognizes mouse SPLUNC1 (Short palate, lung and nasal epithelium clone 1). SPLUNC1, also known as known as BPI fold-containing family member A1 (BPIFA1), is an abundant protein present in the airway lining fluid of healthy humans and mice. It is mainly produced by epithelial cells in the lung and tracheobronchial region, the submucosal glands of the trachea, the bronchi, and in specific granules of neutrophils. The protein displays antibacterial activity against Gram-negative bacteria such as P. aeruginosa and is thought to be involved in inflammatory responses to irritants in the upper airways. Additionally, it may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Its expression is modulated in multiple lung diseases including cystic fibrosis, COPD, respiratory malignancies, and idiopathic pulmonary fibrosis. However, the precise role of SPLUNC1 in pulmonary pathogenesis remains to be elucidated.

Specifications

Catalog number HP8043
Product type Polyclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application P, W
Application Notes W: A reduced sample treatment and SDS-Page was used. The band size is 50 kDa (Ref.2). IHC-P: Tissue sections were pretreated with tri-sodium citrate buffer for 8 minutes in a microwave oven followed by rinsing in PBS. Tissue sections were fixed in 3 % H2O2 in methanol for 20 minutes (Ref.1)
Use For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
Immunogen Generated against epitope 31-46 (GPPLPLNQGPPLPLNQ)
Isotype Rabbit Ig
Species Rabbit
Cross reactivity Rabbit - Yes
Alias BPI fold-containing family member A1, BPIFA1
Positive Control Nasopharyngeal tissues
References 1. Musa M et al; Differential localisation of BPIFA1 (SPLUNC1) and BPIFB1 (LPLUNC1) in the nasal and oral cavities of mice. Cell Tissue Res. 2012, 350:455
2. Bingle L et al; BPIFB1 (LPLUNC1) is upregulated in cystic fibrosis lung disease. Histochemistry and Cell Biology 2012, 138: 749
3. Tadokoro T et al; IL-6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells. PNAS 2014, 111:3641
4. Chen H et al; Airway epithelial progenitors are region specific and show differential responses to bleomycin-induced lung injury. Stem cells 2012, 30:1948
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease Pulmonology, Tumor immunology