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Elafin, Human, pAb

Catalog #: HP9025

Quantity: 100 µg

Availability: In stock

Description: Elafin, Human, pAb

Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria.

Specifications

Catalog number HP9025
Product type Polyclonal antibodies
Quantity 100 µg
Formulation 1 ml diluted goat serum in PBS containing 0.02% sodium azide.
Application IA, P, W
Use For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:100. For Western blotting it is recommended to use non-reducing conditions.
Isotype Goat Ig
Species Human
References 1. Alkemade, J et al; Demonstration of skin-derived antileukoproteinase (SKALP) and its target enzyme human leukocyte elastase in squamous carcinoma. J Pathol 1994, 174: 121
2. Bergen van, H et al; Expression of SKALP/elafin during wound healing in human skin. Arch Dermatol Res 1996, 288: 458
3. Pfundt, R et al; Constitutive and inducible expression of SKALP/elafin provides anti-elastase defense in human epithelia. J Clin Invest 1996, 98: 1389
4. Schalkwijk, J et al; Localization of SKALP/elafin. J Invest Dermatol 1993, 100: 390
5. Schalkwijk, J et al; The trappin gene family: proteins defined by an N-terminal transglutaminase substrate domain and a c-terminal four-disulphide core. Biochem J 1999, 340: 569
6. Wiedow, O et al; Elafin: an elastase-specific inhibitor of human skin. J Biol Chem 1990, 265: 14791
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Autoimmunity, Gastroenterology