Discount Human LBP ELISA and Human sCD14 ELISA

Friday - June 24, 2011

Valid form 1st of July until 31st of December

	Discount	Promo code
1 kit	20%	HA1114
3 kits	three for two	HA1115

Cat.#HK315 Human LBP ELISA
Cat.#HK320 Human sCD14 ELISA

Introduction sCD14 (Cat.# HK320)

The protein encoded by this gene is a component of the innate immune system. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 KDa) or is directly secreted from intracellular vesicles (56 KDa). CD14 takes its name from its inclusion in the cluster of differentiation group of cell surface marker proteins. CD14 was the first described pattern recognition receptor. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14 is expressed mainly by macrophages and (at 10 times lesser extent) by neutrophil granulocytes. It is also expressed by dendritic cells. A soluble form sCD14 is secreted by the liver and monocytes and is sufficient in low concentrations to confer LPS-responsiveness to cells that otherwise do not express CD14. sCD14 is also present in human milk, where it is believed to regulate microbial growth in the infant gut. CD14 has been shown to interact with Lipopolysaccharide-binding protein.

References

sCD14

§ Decreased circulating lactoferrin in insulin resistance and altered glucose tolerance as a possible marker of neutrophil dysfunction in type 2 diabetes. JM Moreno-Navarrete, FJ Ortega… - Journal of Clinical …, 2009 ... The Journal of Clinical Endocrinology & Metabolism Vol. 94, No. 10 4036-4044 plasma bactericidal permeability increasing (BPI) protein levels were measured by human BPI ELISA kit (HyCult Biotechnology bv, PB Uden, The Netherlands); serum soluble CD14 (sCD14) levels were measured by human sCD14 ELISA kit (HyCult Biotechnology); serum LPS ...

§ Serum sCD14, polymorphism of CD14− 260 and periodontal infection T Raunio, M Knuuttila, R Karttunen, O Vainio… - Oral …, 2009 ... Oral Diseases Volume 15, Issue 7, pages 484–489, October 2009 Serum sCD14 concentrations of the samples were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) (Human sCD14 Elisa test kit; Hycult biotechnology, Uden, the Netherlands). The ...

§ Chlamydial lipopolysaccharide (cLPS) is present in atherosclerotic and aneurysmal arterial wall--cLPS levels depend on disease manifestation P Vikatmaa, T Lajunen, TS Ikonen… - Cardiovascular …, 2010... Cardiovascular Pathology, Volume 19, Issue 1, Pages 48-54 ……..samples. Serum LBP was measured with an Hbt Human LBP ELISA test kit, HK315, and sCD14 was measured with an Hbt Human sCD14 ELISA test kit, HK320 (HyCult Biotechnology), according to the manufacturer's instructions. ...

§ Clinical experience with lipopolysaccharide adsorber in cancer patients with severe sepsis and septic shock E Gromova, M Kisselevskiy, N Anisimova… - Critical Care, 2010... Critical Care 2010, 14(Suppl 1):P409 The patient's blood was taken before and after adsorption, then the serum was separated and stored at -70°C. We determined the LPS concentration by LAL test, lipopolysaccharide-binding protein (LBP), sIL-1 RII and sCD14 by ELISA kits (Hycult Biotechnology, the Netherlands ...

§ Lipopolysaccharide-binding protein and CD14 are increased in the bronchoalveolar lavage fluid of smokers V Regueiro, MA Campos, P Morey… - European ... ERJ February 1, 2009 vol. 33 no. 2 273-281 These were found to be culture negative in all cases. LBP, sCD14 and interleukin-8 determinations LBP and sCD14 levels were determined in the unconcentrated BALF after removing cells by centrifugation using ELISA kits (HyCult Biotechnology, Uden, the Netherlands). ...

§ Emulsified lipids increase endotoxemia: possible role in early postprandial low-grade inflammation F Laugerette, C Vors, A Géloën… - The Journal of …, 2010 JNB Volume 22, Issue 1, Pages 53-59 (January 2011)... cluster of differentiation 14 (sCD14) and, which is induced during septic diseases and can also be considered as a marker of endotoxin in plasma. Plasma samples were assayed using a sandwich ELISA (Hycult Biotechnology, Netherlands ...

§ Endotoxin-directed innate immunity in tracheal aspirates of mechanically ventilated human neonates KE NATHE, R PARAD, LJ VAN MARTER… - Pediatric …, 2009 - Pediatric Research: Volume 66 - Issue 2 - pp 191-196 .. 20 mins RT) with anti-BPI MAb (mouse IgG1(), clone HM2042; HyCult Biotechnology, Uden ... Figure 9. Positive correlation of extracellular BPI, LBP, and sCD14 in TA supernatants. ELISA measure of extracellular BPI, LBP, and sCD14 in TA supernatants reveals that extracellular ...

§ Expression of Toll-Like Receptors 2 and 4 is Upregulated During Hospital Admission in Traumatic Patients: Lack of Correlation With Blunted Innate Immune …J Pérez-Bárcena, V Regueiro, C Crespí… - Annals of …, 2010 -Annals of Surgery: Volume 251 - Issue 3 - pp 521-527 ... pg/mL for IL-10. LBP and soluble CD14 (sCD14) in serum were determined using a commercial ELISA kit (Hycult biotechnology). The assay sensitivity was 2 pg/mL for LBP or sCD14. Phagocytosis To determine the phagocytic ...

Introduction LBP (Cat.# HK315)

LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Finally, this gene is found on chromosome 20, immediately downstream of the BPI gene. Lipopolysaccharide-binding protein has been shown to interact with CD14, TLR2, TLR4 and the co-receptor MD-2. The host response to bacterial compounds is regulated and modulated by certain serum proteins and cellular receptor molecules. LBP and soluble CD14, both able to bind LPS, are present in serum in high quantities. LBP is an acute-phase protein synthesized in the liver, the concentration of which rises dramatically during systemic infection and the acute-phase response. It monomerizes LPS vesicles and transports LPS to the CD14 part of the cellular LPS receptor enabling inflammatory responses, such as TNF-α synthesis. LPS effects can be blocked by a range of inhibitors including polymyxin B, a polypeptide known to bind lipid A, the active moiety of LPS.

References

LBP

§ Chlamydial lipopolysaccharide (cLPS) is present in atherosclerotic and aneurysmal arterial wall--cLPS levels depend on disease manifestation P Vikatmaa, T Lajunen, TS Ikonen… - Cardiovascular …, 2010 Cardiovascular Pathology, Volume 19, Issue 1, Pages 48-54. Serum LBP was measured with an Hbt Human LBP ELISA test kit, HK315, and sCD14 was measured with an Hbt Human sCD14 ELISA test kit, HK320 (HyCult Biotechnology), according to the manufacturer's instructions. ...

§ Complement Factor H is expressed in adipose tissue in association with insulin resistance JM Moreno-Navarrete, R Martínez-Barricarte… - Diabetes, 2010... Diabetes January 2010 vol. 59 no. 1 200-209 Serum-soluble tumor necrosis factor-α receptor-2 (sTNFR2); serum lipopolysaccharide binding protein (LBP) levels were measured by Human LBP ELISA kit (HyCult biotechnology, PB ...

§ Gastrointestinal Nematode Infection Exacerbates Malaria-Induced Liver Pathology H Helmby - The Journal of Immunology, 2009 - vol. 182 no. 9 5663-5671... LBP in sera was analyzed by a commercial ELISA kit (Hycult Biotechnology) according to the manufacturer's instructions. . ...

§ Metabolic endotoxemia and saturated fat contribute to circulating NGAL concentrations in subjects with insulin resistance JM Moreno-Navarrete, M Manco, J Ibáñez… - International Journal of …, 2009 -International Journal of Obesity 34, 240-249serum LBP levels were measured using Human LBP ELISA Kit (HyCult Biotechnology bv, PB Uden, The ...

§ Quantitative indices of dynamics in concentrations of lipopolysaccharide-binding protein (LBP) as prognostic factors in severe sepsis/septic shock patients-comparison …M Mierzchala, M Krzystek-Korpacka, A Gamian… - Clinical Biochemistry in press, 2011... Serum LBP was measured by a commercially available enzyme-linked immunosorbent assay (ELISA) and endotoxin (lipopolysaccharide; LPS) using the LAL Chromogenic Endpoint Assay purchased from Hycult biotechnology (Uden, Netherlands); according to manufacturer s ...

§ Proteomic evidence of bacterial peptide translocation in afebrile patients with cirrhosis and ascites R Caño, L Llanos, P Zapater, S Pascual… - Journal of molecular medicine, Volume 88, Number 5, 487-495, 2010 - Human lipopolysaccharide-binding protein (LBP) ELISA kit (HyCult Biotechnology, Uden, The Netherlands) was used to evaluate AF LBP levels in all patients. All samples were tested in triplicate ...

§ Decreased circulating lactoferrin in insulin resistance and altered glucose tolerance as a possible marker of neutrophil dysfunction in type 2 diabetes v Moreno-Navarrete, FJ Ortega… - Journal of Clinical …, 2009... The Journal of Clinical Endocrinology & Metabolism Vol. 94, No. 10 4036-4044 ELISA kit (HyCult Biotechnology bv, PB Uden, The Netherlands); serum soluble CD14 (sCD14) levels were measured by human sCD14 ELISA kit (HyCult Biotechnology); serum LPS binding protein (LBP) levels were measured by human LBP ELISA kit (HyCult Biotechnology). ...

§ Assessment of cholesteryl ester transfer protein inhibitors for interaction with proteins involved in the immune response to infection RW Clark, D Cunningham, Y Cong… - Journal of lipid …, 2010 – ASBMB ... The Journal of Lipid Research, 51, 967-974. Human LBP and BPI ELISA kits were purchasd from Hycult Biotechnolgy. Biotin LPS (Escherichia coli O111:B4 coupled through oxidized carbohydrate to hydrazide LC biotin) used for SPR experiments was from