Technical datasheet
Description
The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered FITC conjugated antibody solution in PBS, containing 0.02% sodium azide and 1% bovine serum albumin.
Species
Rat IgG2b
Application
The FITC conjugated monoclonal antibody 440c can be used for immunohistology on frozen sections and flow cytometry.
Use
For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Blasius, A et al; Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood 2006, 107: 2474
- Blasius, A et al; A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood 2004, 103: 4201
- Colonna, M et al; Plasmacytoid dendritic cells in immunity. Nat Immunol 2004, 5: 1219
- Seth, S et al; CCR7 Essentially Contributes to the Homing of Plasmacytoid Dendritic Cells to Lymph Nodes under Steady-State As Well As Inflammatory Conditions. J Immunol 2011, 186: 3364
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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