Technical datasheet
Description
The monoclonal antibody KP1 reacts with human CD68, a heavily glycosylated transmembrane protein of 110 kD. CD68 is highly expressed by monocytes and tissue macrophages, and at low levels by dendritic cells. The class D scavenger receptor CD68 plays a significant role in oxidized low-density lipoprotein (OxLDL) uptake by activated macrophages and contributes to the formation of foam cells in atherosclerotic lesions. Since scavenger receptors also function as pattern recognition receptors for a wide variety of pathogens, CD68 may have a potential role in host defense. Monoclonal antibody KP1 is widely used to detect macrophages in paraffin sections. KP1 is of considerable value for studying disorders of the monocyte/macrophage system, including both reactive and neoplastic states (such as true histiocytic proliferations).
Formulation
Lyophilized tissue culture supernatant containing 15 mM sodium azide. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).
Species
Mouse IgG1
Application
The monoclonal antibody KP1 can be used for flow cytometry and immunohistology on frozen and paraffin sections. Furthermore, the monoclonal antibody KP1 is useful for Western blot and immuno precipitation.
Use
For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Storage and stability
Lyophilized product should be stored at 4°C. Store stock solution in aliquots at -20°C. Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
References
- Pulford K, et al; KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol 1989, 42: 414
- Doussis T, et al; CD68 reactivity of non-macrophage derived tumours in cytological specimens. J Clin Pathol 1993, 46: 334
- Ramprasad M, et al; Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein. Proc Natl Acad Sci 1996, 93: 14833
- Austin, A et al; Responses to free lipopolysaccharide and Escherichia coli by normal human intestinal macrophages, following their migration out of the lamina propria. Scand J Immunol 2005, 61: 575
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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