Technical datasheet
Description
The monoclonal antibody 2A11 recognizes mouse β-glucan receptor, also known as dectin-1. Dectin-1 is a small type II transmembrane receptor containing one lectin-like carbohydrate recognition domain. This domain recognizes β1,3- and/or β1,6-linked glucans, intact yeast and an immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic tail. β-Glucan is one of the most abundant polysaccharides in fungal pathogens. By recognizing β-glucan, dectin-1 has a fundamental function in antifungal immunity. Dectin-1 is a signaling non–Toll-like pattern-recognition receptor required for the induction of protective immune responses. Dectin-1 is responsible for the β-glucan-dependent, nonopsonic recognition of zymosan by primary macrophages. Zymosan is a yeast derived particle composed principally of polysaccharides, of which β-glucan and mannan are the major constituents. In vivo administration of zymosan, or purified soluble β-glucans, has a number of desirable effects on immune function, including the ability to confer resistance to tumors and various infections.
The receptor is highly expressed on macrophages and neutrophils and to a lesser extent on dendritic cells and a subpopulation of T cells. Dectin-1 participates with the complement receptor CR3 in a host proinflammatory cytokine response to a fungal pathogen. The monoclonal antibody 2A11 recognizes an extracellular epitope on dectin-1.
Immunogen
NIH 3T3 cells transduced with full-length Dectin-1
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Rat IgG2b
Application
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F5
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FC1-4,6,7
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FS1,2,4,7
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IA
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IF
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IP1
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P
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W
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Yes
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No
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N.D.
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N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot
Application notes
FC: Antibody 2A11 stains the extracellular domain of mouse dectin-1. For intracellular staining mouse macrophages were permeabilized with IntraPrep. The macrophages were incubated with 1 µg/ml antibody for 1 hr at 4 °C. (Ref.4)
IHC-F: Tissue sections were frozen in Tissue-Tek and fixed in acetone. As positive control lymph nodes were used (Ref.5).
FS: Antibody 2A11 functions as inhibitor of phagocytosis by zymosan. The antibody was functionally tested by endocytosis assays. The biological activity of the antibody can be defined as the percentage phagocytosis inhibition (Ref.7).
Use
For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
Aliases
beta-glucan receptor
Positive control
Mouse primary macrophages
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
References
1. Brown, G et al; Dectin-1 is a major β-glucan receptor on macrophages. J Exp Med 2002, 196: 407
2. Taylor, P et al; The β-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J Immunol 2002, 169: 3876
3. Brown, G et al; Dectin-1 mediates the biological effects of β-glucans. J Exp Med 2003, 197: 1119
4. Diniz, S et al; PTX3 function as an opsonin for the dectin-1-dependent internalization of zymosan by macrophages. J Leukoc Biol 2004, 75: 649
5. Dupasquier, M et al; The dermal microenvironment induces the expression of the alternative activation marker CD301/mMGL in mononuclear phagocytes, independent of IL-4/IL-13 signaling. J Leukoc Biol 2006, 80: 838
6. Huysamen, C et al; CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes. J Biol Chem 2008, 383: 16693
7. Lin, J-S et al; Distinct roles of complement receptor 3, Dectin-1, and sialic acids in murine macrophage interaction with Histoplasma yeast. J Leukoc Biol 2010, 88: 1
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
References
1. Brown, G et al; Dectin-1 is a major β-glucan receptor on macrophages. J Exp Med 2002, 196: 407
2. Taylor, P et al; The β-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J Immunol 2002, 169: 3876
3. Brown, G et al; Dectin-1 mediates the biological effects of β-glucans. J Exp Med 2003, 197: 1119
4. Diniz, S et al; PTX3 function as an opsonin for the dectin-1-dependent internalization of zymosan by macrophages. J Leukoc Biol 2004, 75: 649
5. Dupasquier, M et al; The dermal microenvironment induces the expression of the alternative activation marker CD301/mMGL in mononuclear phagocytes, independent of IL-4/IL-13 signaling. J Leukoc Biol 2006, 80: 838
6. Huysamen, C et al; CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes. J Biol Chem 2008, 383: 16693
7. Lin, J-S et al; Distinct roles of complement receptor 3, Dectin-1, and sialic acids in murine macrophage interaction with Histoplasma yeast. J Leukoc Biol 2010, 88: 1