Technical datasheet
Description
The monoclonal antibody ER-MP23 specifically reacts with the macrophage galactose-specific lectin (MGL), a 38 kDa single chain surface glycoprotein. MGL is found on murine mature macrophages in the connective tissue neighboring epithelia of e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. MGL is present on the surface of connective tissue macrophages and their precursor cells in bone marrow. Also, MGL is expressed in macrophage cell lines (e.g. J774-1.6, RAW309Cr.1 and WR19M.1). Expression levels of MGL are increased in mature macrophages. The antigen is co expressed with the antigen recognized by monoclonal antibody BM8 (HM1066).
The monoclonal antibody ER-MP23 has been raised after immunization of rats with mouse macrophage cell lines.
Blocking studies demonstrated that the monoclonal antibody ER-MP23 is able to block mouse MGL.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Rat IgG2a
Application
The monoclonal antibody ER-MP23 can be used for immunohistology on frozen sections. Furthermore, the monoclonal antibody is useful for flow cytometry and for studies on inhibition of biological activity of the mouse macrophage galactose-specific lectin (MGL).
Use
For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For blocking studies in vitro, dilutions have to be made according to the amounts of MGL to be inactivated. The antigen is glutaraldehyde (0.05%) resistant.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Jansen, A et al; Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulits and beta-cell destruction in NOD mice. Diabetes 1994, 43: 667
- Rosmalen, J et al; Islet abnormalities associated with an early influx of dendritic cells and macrophages in NOD and NODscid mice. Lab Invest 2000, 80: 769
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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